Robert E. Pruitt

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We have identified a number of genes of the flowering plant Arabidopsis thaliana that are abundantly expressed during embryogenesis. In this paper we discuss four of these genes, which comprise a gene family: complete genomic nucleotide sequence of two of the genes and partial sequence of the other two shows that they are all homologous to the 12S globulin(More)
Escherichia coli O157:H7 has been associated with numerous outbreaks involving fresh produce. Previous studies have shown that bacteria can be internalized within plant tissue and that this can be a source of protection from antimicrobial chemicals and environmental conditions. However, the types of tissue and cellular locations the bacteria occupy in the(More)
The effects of ascorbate deficiency on carnitine biosynthesis was investigated in young male guinea pigs. Liver and kidney carnitine levels were not affected by the deficiency, but scorbutic animals had 50% less carnitine in heart and skeletal muscle than control animals. Labeled carnitine precursors, 6-N-tri-methyl-L-lysine and 4-N-trimethylaminobutyrate,(More)
The last decade provided the plant science community with the complete genome sequence of Arabidopsis thaliana and rice, tools to investigate the function of potentially every plant gene, methods to dissect virtually any aspect of the plant life cycle, and a wealth of information on gene expression and protein function. Focusing on Arabidopsis as a model(More)
The muscarinic receptors coupled to pepsinogen secretion on isolated frog esophageal peptic cells have been characterized using functional and radioligand binding techniques. N-[3H]methylscopolamine [( 3H]NMS) binding to intact cells was complex and indicative of a high affinity, low capacity site and a high capacity uptake site. Binding to the high(More)
Bach, Christopher E., Purdue University, May 2015. Influence and characterization of microbial contaminants associated with the FDA BAM method used to detect Listeria monocytogenes from Romaine lettuce. Major Professor: Robert E. Pruitt. Over the past few decades in the US, fresh produce commodities have become increasingly prevalent vehicles for the(More)
Science 234, 464 (1986); K. A. Feldmann and M. D. Marks, Mol. Gen. Genet. 208, 1 (1987). 6. E. M. Meyerowitz and R. E. Pruitt, Science 229, 1214 (1985). 7. G. R. Fink, Genetics 149, 473 (1998). 8. C. Koncz, N.-H. Chua, J. Schell, Eds., Methods in Arabidopsis Research (World Scientific, River Edge, NJ, 1992); J. M. Martinez-Zapater and J. Salinas, Eds.,(More)
Salmonella serovars have been associated with the majority of foodborne illness outbreaks involving tomatoes, and E. coli O157:H7 has caused outbreaks involving other fresh produce. Contamination by both pathogens has been thought to originate from all points of the growing and distribution process. To determine if Salmonella serovar Typhimurium and E. coli(More)
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