Robert D. Campo

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The purposes of these experiments were to study the biosynthetic and postbiosynthetic relationships between proteoglycans in noncalcified growth cartilage and calcified cartilage in metaphysis from the costochondral junctions of immature rabbits. Based onin vivo experiments in which 35 S-sodium sulfate was injected into rabbits, it is shown that(More)
Tibiae and humeri were removed from suckling rats at intervals of time after intraperitoneal injection of C(14)-L-phenylalanine, C(14)-L-leucine, S(35)-sulfate, or Ca(45) Cl(2). Autoradiograms of sections of the bones were prepared. Ca(45) was removed from sections treated with dilute acetic acid; neither the concentration of S(35) nor that of C(14) was(More)
The localizations of resistant proteoglycans (RPGs) in the epiphyseal plates of rats, dogs, and humans are similar. In the epiphyseal plates from young rats, dogs, and humans, the RPGs form a stratum at the junction of the zones of resting and proliferating cells. Non-calcified cartilage RPGs are associated with cells which have the potential for(More)
The proteoglycans of cartilage occur in a form which is readily extracted (soluble) and in form which is relatively difficult to extract (resistant). Following the extraction of the soluble proteoglycans from slices of epiphyses from young rats, the distribution of the resistant proteoglycans are visualized by staining with toluidine blue. Daily(More)
An understanding of the interrelationships between cells and the intercellular substance of cartilages may help resolve the question of why some cartilages ossify and others do not. Thus far, in autoradiograms of growing bones, S%ulfate has been seen concentrated initially in chondrocytes (l-3), from which it is extruded into the matrix (1)) and thence, a(More)
Slices of fresh ovine and bovine epiphyseal cartilages swell following extraction in 0.05 M disodium ethylenediaminetetraacetate (EDTA) in Tris buffer, pH 5.8 and 7.4, at 4° and 37°. The swelling is strikingly visible to the unaided eye and is most pronounced in the growth plate region of the epiphysis. Other chelators—ethyleneglycolbis(β-aminoethyl(More)
Soluble proteoglycans (SPG) were extracted from bovine (BCC) and human (HCC) costal cartilages by the dissociative method using 4 M guanidinium chloride (GuHCl). Proteoglycans which are resistant to extraction (RPG) were obtained following collagenase digestion or hydroxylamine treatment of the cartilage residues. Similarly, SPG were extracted from bovine(More)