Robert Burton

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Malaria is a major public health problem in sub-Saharan African countries including Ethiopia. Early and accurate diagnosis followed by prompt and effective treatment is among the various tools available for prevention, control and elimination of malaria. This study aimed to evaluate the performance of non-instrumented nucleic acid amplification(More)
Highly sensitive and field deployable molecular diagnostic tools are critically needed for detecting submicroscopic, yet transmissible levels of malaria parasites prevalent in malaria endemic countries worldwide. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and evaluated in comparison with thick blood smear(More)
In recent years there has been some concern for the lack of accuracy in mathematical software provided by computer manufacturers. Of particular concern is the inaccuracy of the subprograms for the elementary mathematical functions. These functions are used extensively in many applications and must be both accurate and efficient. In response to user concern(More) • Vol. 81 No. 10 October 2004 • Journal of Chemical Education 1457 It has been our experience that students have a real interest in compounds derived from natural sources that have biological activity. Unique examples pique their curiosity and provide a connection between “classroom chemistry” and the real world. One such class of(More)
Microscopy and field adaptable rapid diagnostic tests (RDTs) are not sensitive and specific in certain conditions such as poor training of microscopists, lack of electricity, or lower sensitivity in the detection of non-falciparum malaria. More sensitive point-of-care testing (POCT) would reduce delays in diagnosis and initiation of therapy. In the current(More)
Malaria programs aimed at eliminating Plasmodium falciparum (Pf) incorporate active infection detection strategies to target the subclinical transmission reservoir. Currently available immunochromatographic lateral flow tests lack the sensitivity required for Pf malaria elimination; the limit of detection (LOD) of these rapid diagnostic tests (RDTs) is(More)
Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in(More)