Robert Bourdage

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Experiments were conducted in vitro on ovine small luteal cells to evaluate their steroidogenic response to ovine luteinizing hormone (oLH) and human chorionic gonadotropin (hCG) administered continuously throughout the experimental period or as a 15-min pulse. Both oLH and hCG stimulated a significant increase in progesterone secretion (P less than 0.001)(More)
Distributions of embryos and 500-micron diameter microspheres were measured in the cleared oviducts of 32 rabbits at 8 intervals post coitus (pc) and normalized to percentage of isthmic length. By 18 h pc, 46% of the embryos had entered the isthmus and were denuded of the cumulus, while 55% of the microspheres had entered the isthmus. By 24 h pc, all(More)
Muscular activity of the oviductal isthmus was recorded in intact awake rabbits with an extraluminal optoelectronic transducer. Telemetered data were collected continuously during the periovulatory period to relate changes in contraction frequency to oviductal transport function. Mean frequencies during estrus ranged from 11.7 to 18.7 contractions/min.(More)
The feasibility of using black polystyrene microspheres as surrogate ova to obtain quantitative information regarding ovum motion in the oviductal isthmus was tested in rabbits. The 175-microns diameter surrogates were instilled into the ampulla at the time of ovulation, and later the oviducts were exposed for direct observation of surrogate movements in(More)
An optoelectronic instrument to record oviductal muscular activity in chronically instrumented animals was evaluated in in vitro and in vivo experiments. The intensity of red light transmitted through the oviduct was modulated by contractions of the oviductal wall producing an optical analog of the mechanical events. Accuracy of the analog was tested by(More)
Microsurgical transection and anastomosis of the left isthmus in 7 rabbits caused fertility to be reduced 40%. Following rebreeding artificial eggs were introduced into the anastomosed and control oviducts. Sixty-three to 65 hours after mating, direct in vivo observations disclosed varied forms of motion of the artificial eggs within the isthmus, including(More)
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