Robert B. Polisky

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The ability of Mycoplasma pneumoniae cells and membranes to affect tetrazolium reduction by hamster trachea organ cultures was evaluated. Uninfected trachea explants reduced 2,3,5-triphenyl tetrazolium chloride (TTC) and nitro-blue tetrazolium when incubated at 37 C in the absence of air. Reduced tetrazolium salts (formazans) were extractable with acetone(More)
The amount of adenosine triphosphate (ATP) in hamster trachea organ cultures was determined with a technique based on light emission from a luciferin/luciferase/ATP reaction. The amount of ATP, expressed as ng per mg dry weight, was consistent in tracheal explants prepared from various animals and changed negligibly when explants were cultivated in vitro(More)
Hamster trachea organ cultures were exposed to isolated membranes of Mycoplasma pneumoniae, PI 1428. Attachment, monitored by the uptake of tritiated membranes, was relatively insensitive to neuraminidase pretreatment, unlike the attachment of viable cells. Membrane attachment was optimal when explants were incubated with 50 to 100 micrograms of membrane(More)
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