Robert A Patton

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Two 4 x 4 Latin square trials (4-wk periods; 16 wk total) were conducted to see whether supplementing rumen-protected Met (RPM; fed as Mepron) would allow feeding less crude protein (CP), thereby reducing urinary N excretion, but without losing production. In trial 1, 24 Holsteins were fed 4 diets as total mixed rations containing [dry matter (DM) basis]:(More)
An incomplete 8 x 8 Latin square trial (4-wk periods; 12 wk total) using 32 multiparous and 16 primiparous Holstein cows was conducted to assess the production response to crude protein (CP), digestible rumen-undegraded protein (RUP), and rumen-protected Met (RPM; fed as Mepron; Degussa Corp., Kennesaw, GA). Diets contained [dry matter (DM) basis] 21%(More)
The effect of ruminally protected Met (RPM) on splanchnic metabolism was measured in 3 primiparous and 3 multiparous Holstein cows. Doses of RPM (0, 36, and 72 g/d) were tested in a replicated 3 x 3 Latin square design, over 3 consecutive 14-d experimental periods. A mixed ration was fed in 12 equal meals per d (average dry matter intake: 17.5 +/- 0.08(More)
A meta-analysis of published studies was used to investigate the effect of rumen-protected methionine (RPM) added to the diets of lactating dairy cattle on dry matter intake, milk production, true milk protein (TMP) production, and milk fat yield. Differences in responses between 2 commonly used RPM products, Mepron (Evonik Industries, Hanau, Germany) and(More)
Humans, monkeys, and rats were trained by a process of successive differentiations to press a bar for at least 1.00 sec but for no longer than 1.27 sec. Initially, animals were reinforced for all responses, then a minimum duration of response was gradually differentiated, below which no responses were reinforced. Finally, a maximum duration of response was(More)
The objective of this experiment was to evaluate the effect of supplementing a metabolizable protein (MP)-deficient diet with rumen-protected (RP) Lys, Met, and specifically His on dairy cow performance. The experiment was conducted for 12 wk with 48 Holstein cows. Following a 2-wk covariate period, cows were blocked by DIM and milk yield and randomly(More)
Multiparous Holstein cows (n = 16) were used in a replicated (n = 4) Latin square design with 2-wk periods to determine whether length of the experimental period chosen within Latin square designs would influence experimental outcomes for performance-related variables. Cows were fed a basal TMR formulated to supply Lys in excess of the predicted(More)
Our objective was to evaluate the potential of rumen epithelium to transport 2-hydroxy-4-(methylthio)-butanoic isopropyl ester (HMBi) using the Ussing chamber technique. Rumen tissues were obtained from a nearby slaughterhouse, separated from the muscle and serosal layer as quickly as possible after exsanguination, placed in buffer, and gassed with 95:5(More)