Robbert van Schie

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A critical event during programmed cell death (PCD) appears to be the acquisition of plasma membrane (PM) changes that allows phagocytes to recognize and engulf these cells before they rupture. The majority of PCD seen in higher organisms exhibits strikingly similar morphological features, and this form of PCD has been termed apoptosis. The nature of the PM(More)
The unmasked underwater hearing sensitivities of an 8-year-old male and a 7-year-old female Steller sea lion were measured in a pool, by using behavioral psychophysics. The animals were trained with positive reinforcement to respond when they detected an acoustic signal and not to respond when they did not. The signals were narrow-band, frequency-modulated(More)
Different classes of receptors for the Fc moiety of IgG (Fc gamma R) have been defined on human monocytes and macrophages: Fc gamma RI, Fc gamma RII, and Fc gamma RIII. All three classes are capable of mediating antibody-dependent cell-mediated cytotoxicity (ADCC). Fc gamma RI, which binds monomeric human IgG (hIgG) with high affinity, was shown an(More)
Human monocytes and macrophages express an isoform of IgG Fc receptor II (Fc gamma RII), Fc gamma RIIa. Two allotypic variants of this receptor could be distinguished with respect to their ability to bind murine (m)IgG1 complexes either strongly or weakly, defined as high-responder (HR) and low-responder (LR), respectively. We investigated the effect of(More)
The maturation and differentiation process of human monocytes and human monocyte-derived macrophage cytotoxicity mediated by receptors for the Fc moiety of immunoglobulin G (Fc gamma Rs) were studied in vitro using the chemotherapeutic drug doxorubicin in combination with the biological response modifier (BRM) recombinant interferon-gamma (rIFN-gamma).(More)
Purified human monocytes were cultured for 2 h, 88 h, and 10 days in plastic tubes (adherent) and for 10 days in Teflon foil bags (non-adherent). Monocytes were incubated with doxorubicin by two short-term exposures (750 or 1500 ng/ml) for 1 h or by continuous exposure (75 ng/ml). Maturation was monitored by measuring the intracellular activity of three(More)
The immature neonatal immune system is thought to result in increased risk of infection. Receptors for the Fc moiety of IgG (Fc gamma R) are important in antibody-mediated clearance of microbes by granulocytes and monocytes/macrophages. As an approach to understanding their role in neonatal life, we have compared the constitutive expression of the three Fc(More)
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