Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity
- Liu HeRobbelien Kooistra M. Harterink
- 15 April 2020
Biology
Using the in vivo model system Caenorhabditis elegans, it is found that the highly conserved UNC-119 protein provides a link between the membrane-associated Ankyrin (UNC-44) and the microtubule-associated CRMP (unC-33) that form a periodic membrane- associated complex that anchors axonal and dendritic microtubules bundles to the cortex.
SNAP-25 gene family members differentially support secretory vesicle fusion
SNAP-25 family members differentially support neuron survival, and dense-core and synaptic vesicle fusion in Snap-25-null mutant neurons, where DCV fusion becomes SNAP-25 dependent.
CAPS-1 requires its C2, PH, MHD1 and DCV domains for dense core vesicle exocytosis in mammalian CNS neurons
- Linda van KeimpemaRobbelien KooistraR. ToonenM. Verhage
- 7 September 2017
Biology, Medicine
All CAPS-1 functional domains are required, and the C2 and PH domain together are not sufficient, for DCV exocytosis in mammalian CNS neurons, showing the relative importance of CAPS for both secretory pathways.
Centrosome‐mediated microtubule remodeling during axon formation in human iPSC‐derived neurons
- Feline W. LindhoutS. Portegies C. Hoogenraad
- 9 April 2021
Biology, Medicine
It is proposed that centrosomes mediate microtubule remodeling during early axon development in human iPSC‐derived neurons, thereby laying the foundation for further axonDevelopment and function.
Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity and functioning
- Liu HeRobbelien Kooistra M. Harterink
- 26 September 2019
Biology
Using the in vivo model system Caenorhabditis elegans, it is found that the highly conserved UNC-119 protein provides a link between the membrane-associated Ankyrin (UNC-44) and the microtubule-associated CRMP (unC-33) that form a periodic membrane- associated complex that anchors axonal and dendritic microtubules bundles to the cell cortex.
Quantitative mapping of transcriptome and proteome dynamics during polarization of human iPSC-derived neurons
- Feline W. LindhoutRobbelien Kooistra C. Hoogenraad
- 21 April 2020
Biology, Medicine
This work cultured human iPSC-derived neurons, characterized early developmental stages, measured electrophysiological responses, and systematically profiled transcriptomic and proteomic dynamics during these steps, finding extensive remodeling of the neuron transcriptome and proteome.