Rob Cuthbertson

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Transgenic mice carrying the murine granulocyte-macrophage colony stimulating factor (GM-CSF) gene expressed from a retroviral promoter exhibit elevated levels of GM-CSF in the serum, urine, peritoneal cavity, and eye. The eyes of transgenic mice are opaque, contain accumulations of macrophages, and develop retinal damage. Similarly, lesions containing(More)
Poly(lactic-co-glycolic) acid (PLGA) bioresorbable microspheres are used for controlled-release drug delivery and are particularly promising for ocular indications. The objective of the current study was to evaluate the pharmacokinetics and safety of a recombinant human monoclonal antibody (rhuMAb HER2) in rabbits after bolus intravitreal administration of(More)
A technique is described for preparing whole retinal vascular digests using distilled water and DNase. This produces a more satisfactory preparation of the mouse retinal vascular bed than "trypsin digestion." Nonetheless, the calculation of endothelial cell/pericyte (E/P) ratios based on cell counts from digest preparations has poor reproducibility due to(More)
Transmission electron microscopy of ultrathin sections was used to measure mouse retinal capillary basement membrane thickness (BMT). BMT increases predictably with age from 50 +/- 9 nm at 6 weeks to 154 +/- 27 nm at 20 months, but is not affected by strain or sex. There is an effect of retinal site, however, with BMT increasing with the radial distance(More)
III Contents Sommaire V Foreword VI Αυαηί-propos VII List of participants VIII Liste des participants POLYPEPTIDES AND OTHER HORMONES POLYPEPTIDES ET AUTRES HORMONES 3 F. Murad Atrial natriuretic peptide, endothelium-dependent vasodilators and nitrovasodilators, role of guanylate cyclase and cyclic GMP in their actions. Le facteur natriurctique atrial et(More)
The molecular events involved in normal and abnormal eye development are of pivotal importance, but the living mammalian embryo during organogenesis is virtually inaccessible to the investigator. We describe a method of whole embryo culture applicable both to rat and to mouse. With these techniques, embryos in vitro develop normally through the crucial(More)
The Ca-sensitive photoprotein aequorin has been used to record repetitive free Ca transients in single rat liver cells responding to either ATP or ADP. The time-course of individual transients is longer when ATP is the agonist. If both agonists operate through the same receptor, then curtailment of the receptors' activity occurs more rapidly when ADP is the(More)
In an absorbing monograph entitled Mécanisme de la physionomie humaine, Duchenne de Boulogne proposed that each emotion has its own specific facial muscle. Employing the most recent and exciting technical inventions of the mid nineteenth century, Duchenne used faradism to stimulate the facial muscles and photography to record their actions. Using electrical(More)
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