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As a discrete approach to genetic regulatory networks, Boolean models provide an essential qualitative description of the structure of interactions among genes and proteins. Boolean models generally assume only two possible states (expressed or not expressed) for each gene or protein in the network, as well as a high level of synchronisation among the(More)
Genes and gene products interact on several levels, forming transcriptional regulatory-, protein interaction-, metabolic- and signal transduction networks. Genetic, biochemical and molecular biology techniques have been used for decades to identify biological interactions; newly developed high-throughput methods now allow for the construction of(More)
In this study, the authors explored the utility of a descriptive and predictive bionetwork model for phospholipase C-coupled calcium signalling pathways, built with non-kinetic experimental information. Boolean models generated from these data yield oscillatory activity patterns for both the endoplasmic reticulum resident inositol-1,4,5-trisphosphate(More)
UNLABELLED "Human error" in anesthesia can be attributed to misleading information from patient monitors or to the physician's failure to recognize a pattern. A graphic representation of monitored data may provide better support for detection, diagnosis, and treatment. We designed a graphic display to show hemodynamic variables. Twenty anesthesiologists(More)
Cells use signaling and regulatory pathways connecting numerous constituents such as DNA, RNA, proteins and small molecules to coordinate multiple functions, allowing them to adapt to changing environments. High-throughput experimental methods enable the measurement of expression levels for thousands of genes and the determination of thousands of(More)
With increasing emergence of multi-drug resistant infections, there is a dire need for new classes of compounds that act through unique mechanisms. In this work, we describe the discovery and optimization of a novel series of inhibitors of bacterial methionine aminopeptidase (MAP). Through a high-throughput screening campaign, one azepinone amide hit was(More)
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