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For the most frequently used two-site model, an exact binding equation is presented in terms of the total ligand concentration. This equation has been extended to analyze the spectroscopic titration experiment where the dilution of protein solution cannot be neglected, the displacement study, and the effect of non-specific binding. Thus, with a non-linear(More)
OBJECTIVE To detect the levels of mir-18a-5p, mir-23a-3p, mir-24-3p, mir-25-3p and TGF-β1 in tumor microenvironment of esophageal squamous cell cancer (ESCC) patients and explore their clinical significances and correlations. METHODS The mRNA expressions of mir-18a-5p, mir-23a-3p, mir-24-3p, mir-25-3p and TGF-β1 were measured by real-time quantitative(More)
Chemokine/chemokine receptor interactions play a critical role in lymphocyte infiltration of tumors. Recent studies suggest that Th17 cells accumulate within many types of tumors, although the mechanisms that control this are unclear. We studied the distribution and phenotypic features of Th17 cells chemokine receptors, as well as the mRNA levels of CCL2,(More)
The unfolding and refolding behavior of the hexadecameric ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from spinach in solutions of guanidine hydrochloride (GdnHCl) was studied. By a number of criteria (enzyme activity, protein fluorescence, circular dichroism), the enzyme was judged to be almost completely unfolded in 6 M GdnHCl. The changes(More)
It has been shown that inactivation of several enzymes precedes overall conformational changes of the enzyme molecules as a whole during denaturation [Tsou (1993) Science, 262, 380-381]. However, the relation between inactivation, loss of allosteric properties of oligomeric enzymes and unfolding of the enzyme molecule during denaturation remain little(More)
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