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Previous work with low passage synchronized human foreskin fibroblast cell populations has indicated that benzo[alpha]pyrene (BP) can induce a carcinogenic event [3]. BP additionally has shown to damage DNA in log-arithmically growing low passage cultures [9]. High passage cells, on the other hand, seem to be refractory to transformation by BP, even though(More)
The uptake of benzo[a]pyrene (BP) by low passage (LP) and high passage (HP) human skin fibroblast cells is followed by its transport into the nucleus as the parent compound. When the LP and HP cells were treated with BP for 24 h and the DNA was isolated and enzymatically digested, several DNA adducts were detected. In both the LP and HP cells a small amount(More)
The polynuclear aromatic hydrocarbons (PAH) benzo[a]pyrene (BP) and the A-ring reduced analogue of 7,12-dimethylbenz[a]anthracene (DMBA), 1,2,3,4-tetrahydro-7,12-dimethylbenz[a]anthracene (TH-DMBA) are carcinogenic to human cells. The unsaturated PAH, DMBA exhibits no carcinogenic activity on human cells as measured by growth in soft agar. The TH-DMBA and(More)
The similarity of the Arrhenius plots relating temperature to messenger RNA (mRNA) transport from intact and membrane-denuded rat liver nuclei demonstrates that the ATP and cytosol-dependent transport is independent of the lipid phase of the nuclear membrane. This temperature dependence of RNA release was confirmed for alpha 2u-globulin mRNA by use of a(More)
The transport of messenger RNA (mRNA) in response to normal adult (35 kDa) and oncofetal (60 kDa) transport factors has been studied in a reconstituted cell-free system. Poly(A)+ mRNA sequences transported by the 35 kDa and 60 kDa transport factors were compared by cDNA:RNA hybridization kinetics. Heterologous hybridization reactions indicated that a(More)
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