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Real-time polymerase chain reaction (PCR) is a frequently used technique in molecular diagnostics. To date, practical guidelines for the complete process of optimization and validation of commercial and in-house developed molecular diagnostic methods are scare. Therefore, we propose a practical guiding principle for the optimization and validation of… (More)
The Porsolt swim test was used to study the role of glucocorticoids in a simple learning paradigm. Two experiments were performed. In the first experiment, the stressfulness of the situation was manipulated by testing control, sham-operated and adrenalectomized mice at different water temperatures (20 degrees C, 25 degrees C, 30 degrees C and 35 degrees C).… (More)
OBJECTIVE To measure the prognostic value of clinical, anthropometric, and biological indicators of protein energy malnutrition in hospitalised children. DESIGN Hospital based follow up study from admission to discharge or death of a cohort of children. SETTING-Paediatric hospital in Zaire. SUBJECTS 1129 children consecutively admitted between August… (More)
Night blindness and optic neuropathy were the presenting symptoms of an iatrogenic malabsorption syndrome in a 64-year old female. This case illustrates the necessity of lifelong vitamin supplementation after biliopancreatic bypass for morbid obesity.
Central retinal artery occlusion can be caused by excessive extrinsic pressure on the eyeball during surgery. It is a well-known and severe complication after elective back surgery. The authors report a case of central retinal artery occlusion in an adult after posterior lumbar fusion in which a rectangular headrest was used.
Increased cortisol levels have been observed in patients suffering from a number of metabolic and psychiatric disorders. In some of these disorders a causal relationship has been suggested between the increased cortisol secretion and the observed clinical phenomena. Glucocorticoid receptor antagonists which block cortisol effects might have a benefit in… (More)
PURPOSE To examine the hypothesis that removing the center of the posterior capsule would prevent posterior capsular opacification (PCO). SETTING Department of Ophthalmology, University Hospital Antwerp, Belgium. METHODS A posterior continuous curvilinear capsulorhexis (CCC) was done before intraocular lens (IOL) implantation in eyes at risk for PCO… (More)
We examined the hypothesis that removing the center of the posterior capsule would reduce the risk for posterior opacification (PCO). We considered the prevalence of post-operative complications after PCCC like retinal detachment and cystoid macular edema, as found after Nd-YAG laser capsulotomy.