We report a DNA microarray-based method for genome-wide monitoring of competitively grown transformants to identify genes whose overexpression confers a specific cellular phenotype. Whereas transcriptional profiling identifies differentially expressed genes that are correlated with particular aspects of the cellular phenotype, this functional genomics… (More)
A reverse transcription (RT)-PCR technique was developed to analyze global gene regulation in Escherichia coli. A novel combination of primers designed specifically for the start and stop regions of E. coli genes (based on the findings of Fislage et al. [R. Fislage, M. Berceanu, Y. Humboldt, M. Wendt, and H. Oberender, Nucleic Acids Res. 25:1830-1835,… (More)
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Physical heterogeneity in the subsurface poses significant problems for the bioremediation of contaminants, these include: (1) delivery of biological amendments to stimulate bioremediation by hydraulic techniques is limited to soils and sediments with hydraulic conductivities above around 10-7 m s-1 ; and (2) physical heterogeneity imparts controls on… (More)