Richard L Frock

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Genome-wide Translocation Sequencing Reveals Mechanisms of Chromosome Breaks and Rearrangements in B Cells

Roberto Chiarle, Yu Zhang, +12 authors Frederick W Alt
Cell
2011

Whereas chromosomal translocations are common pathogenetic events in cancer, mechanisms that promote them are poorly understood. To elucidate translocation mechanisms in mammalian cells, we developed… (More)

Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila.

W M Deng, Martina Schneider, +4 authors Hannele Ruohola-Baker
Development
2003

The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan… (More)

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

Richard L Frock, Jiazhi Hu, Robin M Meyers, Yu-jui Ho, Erina Kii, Frederick W Alt
Nature Biotechnology
2015

Although great progress has been made in the characterization of the off-target effects of engineered nucleases, sensitive and unbiased genome-wide methods for the detection of off-target cleavage… (More)

Chromosomal Loop Domains Direct the Recombination of Antigen Receptor Genes

Jiazhi Hu, Yu Zhang, +6 authors Frederick W Alt
Cell
2015

RAG initiates antibody V(D)J recombination in developing lymphocytes by generating "on-target" DNA breaks at matched pairs of bona fide recombination signal sequences (RSSs). We employ bait… (More)

Detecting DNA double-stranded breaks in mammalian genomes by linear amplification–mediated high-throughput genome-wide translocation sequencing

Jiazhi Hu, Robin M Meyers, Junchao Dong, Rohit A. Panchakshari, Frederick W Alt, Richard L Frock
Nature Protocols
2016

Unbiased, high-throughput assays for detecting and quantifying DNA double-stranded breaks (DSBs) across the genome in mammalian cells will facilitate basic studies of the mechanisms that generate and… (More)

PAXX and XLF DNA repair factors are functionally redundant in joining DNA breaks in a G1-arrested progenitor B-cell line.

Vipul Surender Kumar, Frederick W Alt, Richard L Frock
Proceedings of the National Academy of Sciences…
2016

Classical nonhomologous end joining (C-NHEJ) is a major mammalian DNA double-strand break (DSB) repair pathway. Core C-NHEJ factors, such as XRCC4, are required for joining DSB intermediates of the… (More)

A-type nuclear lamins, progerias and other degenerative disorders

Erica D. Smith, Brian A. Kudlow, Richard L Frock, Byron K. Kennedy
Mechanisms of Ageing and Development
2005

Nuclear lamins were identified as core nuclear matrix constituents over 20 years ago. They have been ascribed structural roles such as maintaining nuclear integrity and assisting in nuclear envelope… (More)

Orientation-specific RAG activity in chromosomal loop domains contributes to Tcrd V(D)J recombination during T cell development

Lijuan Zhao, Richard L Frock, +4 authors Frederick W Alt
The Journal of experimental medicine
2016

T cell antigen receptor δ (Tcrd) variable region exons are assembled by RAG-initiated V(D)J recombination events in developing γδ thymocytes. Here, we use linear amplification-mediated… (More)

Mechanism of tandem duplication formation in BRCA1 mutant cells

Nicholas A Willis, Richard L Frock, +7 authors Ralph Scully
Nature
2017

Small, approximately 10-kilobase microhomology-mediated tandem duplications are abundant in the genomes of BRCA1-linked but not BRCA2-linked breast cancer. Here we define the mechanism underlying… (More)

Mechanisms of Recurrent Chromosomal Translocations

Richard L Frock, Jiazhi Hu, Frederick W Alt
2015

Chromosomal translocations frequently involve fusion of the ends of two separate DNA double-stranded breaks (DSBs) at distinct genomic locations. Recurrent chromosomal translocations are found in… (More)

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