Richard K J Luke

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The copper resistance of a strain of Escherichia coli isolated from the effluent of a piggery where pigs were fed a diet supplemented with copper sulfate was controlled by a conjugative 78-megadalton plasmid designated pRJ1004. Plasmid pRJ1004 exhibited surface exclusion and incompatibility with standard plasmids belonging to incompatibility groups I1 and(More)
Mutants of Escherichia coli K-12 unable to synthesize the iron-sequestering compound, enterochelin, from 2,3-dihydroxybenzoate have been isolated and divided into three classes on the basis of tests for enzymatic complementation. The genes affected (designated entD, entE, and entF) have been mapped by cotransduction and are located at about minute 14 on the(More)
The virulence of enterotoxigenic Escherichia coli (ETEC) is attributed to their ability to adhere via fimbrial adhesins to specific receptors located on the intestinal mucosa. A novel approach to preventing ETEC induced diarrhoea would be to prevent attachment of ETEC to intestine by proteolytically modifying the receptor attachment sites. This study aimed(More)
Properties of the enzyme which hydrolyses enterochelin (a cyclic trimer of 2,3-dihydroxy-N-benzoyl-L-serine) to 2,3-dihydroxybenzoylserine have been investigated with a view to resolving discrepancies between earlier reports. Enterochelin esterase, previously reported to consists of two components (O'Brien, I.G., Cox, G.B. and Gibson, F. (1971) Biochim.(More)
The copper resistance in Escherichia coli determined by plasmid pRJ1004 is inducible. The level of resistance is proportional to the inducing dose of copper. The level of copper resistance in induced and uninduced cells changes with the growth phase of the culture. Induced resistant cells accumulate less copper than uninduced cells, so that reduced(More)
This investigation is an extension of previous studies on the possible role of intestinal Escherichia coli in sudden infant death syndrome (SIDS) to include the isolation of extraintestinal E. coli. The lungs of 52 and the blood of 144 SIDS infants were cultured and isolates were investigated. E. coli was isolated from about a quarter of post-mortem lung(More)
A limited historical perspective can affect the questions we pose about the sudden infant death syndrome (SIDS) issue. Evidence is presented from the literature that the SIDS rate in Western countries was low prior to World War II and peaked in the 1980s. An analogy is drawn with the trends in the prevalence of some infectious diseases, and questions are(More)
The characterization of Escherichia coli strains isolated from healthy infants under one year of age with respect to O:H serotype, K1 and K5 antigens in two disparate parts of the developed world was the purpose of this investigation. A total of 450 strains were examined, 264 from Berlin and 186 from Melbourne. Of all the 220 different O:H serotypes found,(More)
A mutant of Escherichia coli K-12 unable to form an essential component of the enterochelin-dependent iron transport system has been isolated. This strain carries a mutation in a gene designated fep, mapping close to two genes, entA and entD, concerned with enterochelin synthesis. Strain AN102, which carries the fep(-) allele, accumulates large quantities(More)
AIM To examine the diversity of Escherichia coli serotypes found in the intestinal contents of infants who died of Sudden Infant Death Syndrome (SIDS) compared with that in comparison infants. METHODS AND RESULTS Over the 3-year period, 1989-1991, in South Australia and Victoria (Australia), a total of 687 E. coli isolates from 231 patients with SIDS (348(More)