Richard John Grant

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The impact was studied of buffer pH (5.8, 6.2, and 6.8) on in vitro digestion kinetics of NDF from alfalfa hay, bromegrass hay, corn silage, and alfalfa and bromegrass hays with raw corn starch added to approximate a ration containing 30% NDF. Ash-free NDF was determined at 0, 6, 12, 18, 24, 30, 36, 48, 72, and 96 h of fermentation. Kinetic parameters were(More)
Twelve early lactation Holstein cows (4 fistulated) were used in replicated 4x4 Latin squares with 4-wk periods to determine the effective neutral detergent fiber (NDF) content of wet corn gluten feed and to measure the effect of forage particle size on ruminal mat consistency and passage rate of wet corn gluten feed. Diets were 1) 23.3% NDF (17.4(More)
Total mixed rations containing brown midrib sorghum-sudangrass silage (bmrSS) or corn silage (CS) at either 35 or 45% of dietary dry matter were fed to Holstein dairy cows to determine the effect on lactational performance and nutrient digestibility. Twelve cows were assigned to 1 of 4 diets in replicated 4 x 4 Latin squares with 21-d periods. In vitro 30-h(More)
The objective was to determine whether overstocking during the dry period could alter physiological parameters in dairy cattle associated with cortisol secretion and energy metabolism. Four groups of 10 late-gestation, nonlactating Holstein cows (6 multiparous cows and 4 heifers per group) were exposed to both a control [1 lying stall/cow and 0.67 m of(More)
An in vitro buffering system capable of pH control between pH 5.8 and 6.8 was developed to examine the effect of media pH on disappearance of NDF at various times of fermentation and to assess initially the effect of media pH on kinetics of NDF digestion. The pH conditions selected for evaluation of these buffer systems were 5.8, 6.2, and 6.8. Use of(More)
Three in vitro fermentation experiments were conducted to examine the impact on kinetics of fiber digestion of microminerals and tryptone addition, media reduction, fermentation vessel, CO2 gassing regimen, and buffer type. Alfalfa and bromegrass hays were incubated for 0, 4, 8, 12, 18, 24, 30, 36, 48, 72, and 96 h and analyzed for NDF. Kinetic measures of(More)
The objective of this experiment was to measure ruminal and lactational responses of Holstein dairy cows fed diets containing 3 different starch levels: 17.7 (low; LS), 21.0 (medium; MS), or 24.6% (high; HS). Twelve multiparous cows (118 ± 5 d in milk) were assigned randomly to dietary treatment sequence in a replicated 3 × 3 Latin square design with 3-wk(More)
The objective was to evaluate the association of peripartum concentrations of fecal cortisol metabolites (11,17-dioxoandrostane; 11,17-DOA), plasma cortisol and haptoglobin (Hp), as well as two markers of negative energy balance, non-esterified fatty acid (NEFA) and postpartum β-hydroxybutyrate (BHBA), with milk yield and reproductive performance. Blood and(More)
The US dairy industry has realized tremendous improvements in efficiencies and milk production since the 1940s. During this time, farm and total cow numbers have decreased and average herd size has increased. This intensification, combined with the shift to a largely urban public, has resulted in increased scrutiny of the dairy industry by social and(More)
The association between negative energy balance and health has led to the testing of blood analytes such as nonesterified fatty acids (NEFA) to identify opportunities for improving the management of transition dairy cows. The objective of this study was to evaluate whether prepartum analytes associated with stress (cortisol) or inflammation (haptoglobin)(More)