Richard Craig

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The isolation of high-quality RNA from various tissues (leaves, pedicels, glandular trichomes) of garden geranium (Pelargonium xhortorum) using various published methods is difficult due to numerous oxidizing compounds. A new RNA extraction method was developed through the combination and modification of two separate procedures (Rochester et al., 1986;(More)
In a previous report (1978, Proc. Nat. Acad. Sci. USA 75, 3479-3483), we showed that recipient strains of Streptococcusfaecalis excrete a heat-stable substance (sex pheromone) which induces donor cells carrying certain conjugative plasmids to become adherent, generating the cell-to-cell contact necessary for plasmid transfer. Since donors themselves could(More)
Recipient strains of Streptococcus faecalis excrete multiple, peptide sex pheromones that induce mating responses in donors harboring certain conjugative plasmids. Acquisition of plasmid DNA leads to a "shutting off" of pheromone excretion, and such cells become responsive to exogenous pheromone. Data are presented showing that donors excrete low levels of(More)
Trichome exudate from mite-resistant geraniums (Pelargonium horlorum) was analyzed, principally by mass spectrometry and NMR spectroscopy. The exudate was found to consist of two anacardic acid derivatives,o-pentadecenylsalicylic acid ando-heptadecenylsalicylic acid. Bioassays established a moderate toxicity of these compounds to the two-spotted spider(More)
Bacteria produce a heterogeneous mixture of neutrophil chemotactic agents in culture filtrates. Formylmethionyl peptides have been shown to comprise a significant portion of the chemotactic activity in bacterial culture filtrates; however, not all of the chemotactic agents in bacterial culture filtrates are formylated peptides. To examine whether(More)
The focus of this work is to investigate and quantify the ability of a humanoid ‘hybrid face’ robot to effectively convey emotion to a human observer by mapping their physiological (EEG) response to perceived emotional information. Specifically, we examine the event related response during two implicit emotion recognition experiments to determine the(More)
The garden geranium (Pelargonium xhortorum) has been shown to secrete anacardic acids in the form of a viscous sticky exudate from tall glandular trichomes, and this exudate provides a sticky trap defense against small pest species. The anacardic acids from genetically related pest-resistant and -susceptible plants have been characterized, and resistance(More)
To determine whether latent Epstein-Barr virus (EBV) modifies DNA damage responses in B lymphocytes, cells were treated with agents either producing DNA cross-links and adducts or generating double-strand breaks. The cyclin-dependent kinase inhibitor p21(WAF1) accumulated in mitogen-stimulated primary B cells following exposure to all genotoxins tested. In(More)
The Streptococcus faecalis sex pheromone cPD1, which induces a mating response in cells harboring the conjugative plasmid pPD1, has been isolated and its structure determined. It was found to have a molecular weight of 912, and its amino acid sequence was H-Phe-Leu-Val-Met-Phe-Leu-Ser-Gly-OH. A synthetic octapeptide showed the same biological activity and(More)
Insect-resistant and -susceptible geranium plants (Pelargonium xhortorum) were chemically and morphologically compared to assess the relative importance of resistance components. Glandular and nonglandular trichome density and quantification of trichome exudate (anacardic acids) from inbred resistant and susceptible lines were determined. Three different(More)