Richard A. Gemeinhart

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MicroRNAs (miRNAs) are non-coding endogenous RNAs that direct post-transcriptional regulation of gene expression by several mechanisms. Activity is primarily through binding to the 3' untranslated regions (UTRs) of messenger RNAs (mRNA) resulting in degradation and translation repression. Unlike other small-RNAs, miRNAs do not require perfect base pairing,(More)
To develop novel hybrid paclitaxel (PTX) nanocrystals, in which bioactivatable (MMPSense® 750 FAST) and near infrared (Flamma Fluor® FPR-648) fluorophores are physically incorporated, and to evaluate their anticancer efficacy and diagnostic properties in breast cancer xenograft murine model. The pure and hybrid paclitaxel nanocrystals were prepared by an(More)
In this project, we strived to develop a decellularized human cornea to use as a scaffold for reconstructing the corneal epithelium and anterior stroma. Human cadaver corneas were decellularized by five different methods, including detergent- and nondetergent-based approaches. The success of each method on the removal of cells from the cornea was(More)
To develop a platform for tumor chemotherapy, poly(acrylic acid-co-methyl methacrylate) microparticles have been synthesized. Carboxylate containing monomers were included to complex therapeutic agents, specifically cisplatin. Microparticles were prepared by free radical emulsion polymerization in aqueous media. Particle diameter, zeta-potential, in vitro(More)
Local delivery of cancer chemotherapeutics enables sustained drug levels at the site of action thereby reducing systemic side effects. A novel insertable polymeric drug delivery system for cervical cancer treatment is presented. Cisplatin, the first line of therapy employed for cervical cancers, was incorporated in a poly(ethylene-co-vinyl acetate) (EVAc)(More)
Stimuli-sensitive hydrogels (or smart hydrogels) are hydrogels that swell or shrink in response to small changes in environmental conditions in which they are placed. While the extent of swelling or shrinking may be large, the kinetics of such changes is slow, since the diffusion of water into and out of the hydrogel is a slow process. To obtain fast(More)
Hydrogels have gained acceptance as biomaterials in a wide range of applications, including pharmaceutical formulations, drug delivery, and tissue sealants. However, exploiting the potential of hydrogels as scaffolds for cell transplantation, tissue engineering, and regenerative medicine still remains a challenge due to, in part, scaffold design(More)
The extracellular matrix and extracellular matrix-associated proteins play a major role in growth and differentiation of tissues and organs. To date, few methods have been developed that allow researchers to examine the affect of surface density gradients of adhesion molecules in a controlled manner. Fibroblasts cultured on surfaces with a surface density(More)
Superporous hydrogels (SPHs) swell to a large size in a very short time. In many applications it is preferred to compress SPHs to reduce the overall dimension in the dried state. The effects of compression on the swelling property of SPHs were examined. The swelling property of the compressed SPHs was dependent on the orientation of the SPHs during(More)
Soft-polymer based microparticles are currently being applied in many biomedical applications, ranging from bioimaging and bioassays to drug delivery carriers. As one class of soft-polymers, hydrogels are materials, which can be used for delivering drug cargoes and can be fabricated in controlled sizes. Among the various hydrogel-forming polymers,(More)