Richard A. Albright

Learn More
The structure of the Cro protein from bacteriophage lambda in complex with a 19 base-pair DNA duplex that includes the 17 base-pair consensus operator has been determined at 3.0 A resolution. The structure confirms the large changes in the protein and DNA seen previously in a crystallographically distinct low-resolution structure of the complex and, for the(More)
A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure(More)
Knowledge of the three-dimensional structures of the lambda-Cro and lambda-repressor proteins in complex with DNA has made it possible to evaluate how these proteins discriminate between different operators in phage lambda. As anticipated in previous studies, the helix-turn-helix units of the respective proteins bind in very different alignments. In Cro the(More)
The structure has been determined at 3.0 A resolution of a complex of engineered monomeric Cro repressor with a seven-base pair DNA fragment. Although the sequence of the DNA corresponds to the consensus half-operator that is recognized by each subunit of the wild-type Cro dimer, the complex that is formed in the crystals by the isolated monomer appears to(More)
The crystal structure of an engineered monomer of the lambda Cro repressor shows unexpected expansion of the hydrophobic core of the protein and disorder of the five C-terminal residues [Albright et al. (1996) Biochemistry 35, 735-742]. This structural information has guided the construction of a second generation of monomeric Cro proteins by combinatorial(More)
In an introductory course in computer science, often we are reluctant to giv e more tnan a brier noa to macnine language, usually discussing how the binar y number system is usea to represent various kinds of numbers in a computer . Bu t this is as deep as we get into machine code, so that how programs are handle a by a computer is ratner a "clack-oox(More)
1971 The purpose of this paper is to exhibit a theory of integration which can be used on a large class of topological spaces and which generalizes the theory of integration over the locally compact Hausdorff spaces. The first part of the paper is devoted to the construction of such a theory. An integral is viewed as a positive linear functional having a(More)
  • 1