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We inoculated lodgepole pine (Pinus contorta var. latifolia (Dougl.) Engelm.) with Paenibacillus polymyxa P2b-2R, a diazotrophic bacterium previously isolated from internal stem tissue of a naturally regenerating pine seedling to evaluate biological nitrogen fixation and seedling growth promotion by this microorganism. Seedlings generated from pine seed(More)
Inoculation of western red cedar with Paenibacillus polymyxa P2b-2R, an endophytic diazotroph of a pine, was previously shown to result in biological nitrogen fixation (BNF) in seedlings grown under N-limited conditions, but biomass accumulation was reduced after a 9-month growth period. To determine if the seedling growth reduction was temporary, we(More)
Paenibacillus polymyxa P2b-2R is a bacterium that originated from internal lodgepole pine (Pinus contorta var. latifolia (Dougl.) Engelm.) seedling stem tissue and fixes nitrogen (N) in association with pine and western red cedar (Thuja plicata Donn.). To evaluate endophytic colonization by this microorganism, we generated P. polymyxa P2b-2Rgfp, a green(More)
PURPOSE To characterize intraocular tumors that arise by in situ transformation in the choroid-retinal pigment epithelium (RPE) in transgenic mice bearing the SV40 oncogene under the control of the mouse tyrosinase promoter. METHODS Tumors from TySV40 transgenic mice were characterized in vivo and in vitro by immunohistology, compound microscopy, and(More)
We characterized nif gene structure of Paenibacillus polymyxa strain P2b-2R, an endophytic diazotroph that can provide up to 79 % of foliar N in lodgepole pine through biological nitrogen fixation. We amplified a 388-bp internal nifH gene fragment from P2b-2R using the single specific primer–polymerase chain reaction (SSP-PCR) and performed a Southern blot(More)
Tuberculosis is the most prominent contagious disease and needs the new targets and drugs identification. Target identification and validation is a crucial step in drug discovery process. In Mycobacterium tuberculosis, decaprenyl-phosphoryl-β-d-ribose 2′-oxidase is a potential target for antitubercular chemotherapy. It is encoded by genes dprE1 (Rv3790) and(More)
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