Renato Dulbecco

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Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to(More)
HE T potentialities of bacteriophage genetics have been revealed by the discovery of genetic recombinations among related phage particles infecting the same bacterial cell (DELBR~CK and BAILEY 1946). The complexities of these genetic systems have been further illustrated by the work of HERSHEY and ROTMAN (1948) on a number of different genetic determinants(More)
For the first half century of animal virology, the major problem was lack of a simple method for quantitating infectious virus particles; the only method available at that time was some form or other of the serial-dilution end-point method in animals, all of which were both slow and expensive. Cloned cultured animal cells, which began to be available around(More)
The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing a single cancer-initiating cell with stem cell properties has proven technically difficult because of the scarcity of the cancer stem cells in the tissue of origin and the lack of specific(More)
Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto, which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain(More)
We have studied the progression of breast tumors induced by N-methyl-N-nitrosourea in Wistar/Furst rats during serial passages through isogeneic animals, using characteristic markers previously defined. The tumors contained various types of structure, which evolve from intraductal nodes to duct-like structures and then to invasive strands or cells. Most(More)