Ren-Feng Li

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Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM)(More)
To investigate B cell epitopes on the VP3 of infectious bursal disease virus (IBDV), the VP3 gene of IBDV were subcloned into the pET28a vector to construct expression vector of pETVP3. The recombinant VP3 (rVP3) was expressed in E. coli BL21(DE3) by inducing with IPTG. The expressed VP3 protein was recognized specifically by anti-IBDV antisera in western(More)
Metabolic pathways constructed from sequenced genomes or transcriptomes can not only help predict metabolic phenotypes and discover enzyme but also provide an essential context for understanding comprehensive experimental data and the systematic workings of a cell. In the present study we compared the metabolic pathways of two Actinobacillus(More)
A rapid sample treatment procedure for the gas chromatography-tandem mass spectrometry (GC-MS) determination of 19-nortestosterone (19-NT) in animal tissues has been developed. In our optimized procedures, enzymatic hydrolysis with β-glucuronidase from Escherichia coli was performed in an acetate buffer (pH 5.2, 0.2 mol/L). Next, the homogenate was mixed(More)
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