Reggie A. Fletcher

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The potential role of antioxidant enzymes in protecting maize (Zea mays L.) seedlings from chilling injury was examined by analyzing enzyme activities and isozyme profiles of chilling-susceptible (CO 316) and chilling-tolerant (CO 328) inbreds. Leaf superoxide dismutase (SOD) activity in CO 316 was nearly one-half that of CO 328, in which the high activity(More)
The cucumber cotyledon greening bioassay is frequently used for detecting cytokinins. Beneficial modifications of the original technique included using 5-day-old cucumber (Cucumus sativus L., cv. National Pickling) cotyledons treated with combinations of 40 millimolar KCl and various concentrations of cytokinins. A dark incubation period of 20 hours was(More)
Wheat (Triticum aestivum L.) seeds were imbibed for 18 h in 40 mM KCl solution containing either distilled water or 100 mg l−1 paclobutrazol (PBZ, a triazole) and then air dried. Seeds were germinated in a greenhouse and one set of seedlings was grown under non-stressed conditions. Another three sets were stressed by waterlogging at three different stages(More)
The relationship between gibberellin (GA) levels, height, and stress tolerance was investigated using barley (Hordeum vulgare L.) seedlings, which were exposed to heat stress (50 °C for 3 h) and a free radical generator (Paraquat). Barley cv. Perth seedlings were treated with GA-inhibitors, either a triazole or one of two acylcyclohexanediones. They were(More)
Uniconazole [(E)-(p-chlorophenyl)-4, 4-dimethyl-2-(1,2,4-triazol-1 -yl)-1 -penten-3-ol] belongs to a group of triazoles which, in addition to their fungitoxic and plant growth-regulating (PGR) properties, protect plants from various stresses. Compared to the conventional methods of treatment which include seed, soil drench, and foliar spray, the present(More)
Cotyledons of cucumber plants grown in the dark for 7 days were treated with various concentrations of cytokinins for 14 h and then moved into light. After 3 h the treated cotyledons had up to 450% more chlorophyll than the water controls. This suggests that cytokinins have an important role in the formation of chlorophyll. The increase in chlorophyll level(More)
Hybrid corn (Zea mays L. cv Pioneer 3902) seeds were imbibed either in distilled water (control) or 40 mM KCl solution containing paclobutrazol (P) and ancymidol (A) at 50 and 25 mg 1−1 respectively and air dried. Seedlings were germinated and grown in a controlled environment cabinet with a day/night temperature of 23/18°C and a photoperiod of 16 h with a(More)
In vitro regeneration in chickpea (Cicer arietinum L.) was achieved by direct culture of mature seeds on Murashige and Skoog (MS) medium supplemented with either N-phenyl-N′(-1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ) or N6-benzylaminopurine (BAP). Multiple shoots formed de novo without an intermediary callus phase at the cotyledonary notch region of the(More)