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The duplicated and the highly repetitive nature of the maize genome has historically impeded the development of true single nucleotide polymorphism (SNP) markers in this crop. Recent advances in genome complexity reduction methods coupled with sequencing-by-synthesis technologies permit the implementation of efficient genome-wide SNP discovery in maize. In(More)
A subgenomic library constructed from small Pstl restriction fragments (0.4 to 2.0 kb) yielded 83.18% low-copy clones. Using 17 random genomic and 5 heterologous probes in 65 probe-enzyme combinations, Restriction Fragment Length Polymorphism (RFLP) for nuclear DNA was studied in five desi and five kabuli type chickpea cultivars. Only two clones revealed(More)
Bacillus thuringiensis strains are well known for the production of insecticidal proteins upon sporulation and these proteins are deposited in parasporal crystalline inclusions. The majority of these insect-specific toxins exhibit three domains in the mature toxin sequence. However, other Cry toxins are structurally and evolutionarily unrelated to this(More)
Genetic diversity analysis was carried out in chickpea accessions using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. RFLP analysis using 26 Pst I sub-genomic clones on ten chickpea accessions in 130 probe-enzyme combinations detected polymorphism with only two clones. Pst I clones, CG 141 detected(More)
Three interspecific crosses were developed using Cicer arietinum (ICC 4918) as the female parent and wild Cicer species [C. reticulatum - JM 2100, JM 2106 and C. echinospermum - ICCW 44] as the male parent. Cicer arietinum (ICC 4918) × C. reticulatum (JM 2100) cross produced the largest number of F2 plants and was chosen for linkage mapping using Random(More)
The range and mean protein content of autotetraploids of high-lysine Notch-2 mutants of barley were consistently higher than the diploids in C3 and C4 generations of colchicine treated seeds. Amino acid analysis of whole grain meal of diploid Notch-2 and one strain of its autotetraploid revealed differences in the amino acid composition. The proportion of(More)
1. The length of the chromosome complements of T. monococcum, Ae. speltoides var. ligustica and Ae. squarrosa, the donors of the A, B and D genomes respectively of T. aestivum, varied in the ratio 1.0 ∶ 1.24 ∶ 0.76. The proportion of the total lengths of the chromosomes belonging to the A, B and D genomes in the bread wheat variety Chinese Spring, as(More)
A Feulgen procedure was followed for identifying the somatic chromosomes of cucumber (C. sativus L.). The chromosomes were differentiated into dark and light banded regions facilitating the precise karyotyping of somatic complement. Pre-treatment temperature was found to be not critical for producing Feulgen bands in cucumber.
In Brassica juncea, segregation of 44 RFLP markers generated by homologous genomic DNA clones as probes was studied in a F2 population obtained from an intervarietal cross. Linkage relationship among the markers was established using computer package ‘MAPMAKER’. Twenty five of the markers could be arranged in nine linkage groups, covering a total of 243.3(More)