Rebecca K. Lau

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N6-methyldeoxyadenine (6mA) is a noncanonical DNA base modification present at low levels in plant and animal genomes, but its prevalence and association with genome function in other eukaryotic lineages remains poorly understood. Here we report that abundant 6mA is associated with transcriptionally active genes in early-diverging fungal lineages. Using(More)
The class 4 P-type ATPases ("flippases") maintain membrane asymmetry by translocating phosphatidylethanolamine and phosphatidylserine from the outer leaflet to the cytosolic leaflet of the plasma membrane. In Saccharomyces cerevisiae, five related gene products (Dnf1, Dnf2, Dnf3, Drs2, and Neo1) are implicated in flipping of phosphatidylethanolamine,(More)
Cyanobacteria are important primary producers of organic matter in diverse environments on a global scale. While mechanisms of CO2 fixation are well understood, the distribution of the flow of fixed organic carbon within individual cells and complex microbial communities is less well characterized. To obtain a general overview of metabolism, we describe the(More)
Mixed cultures of different microbial species are increasingly being used to carry out a specific biochemical function in lieu of engineering a single microbe to do the same task. However, knowing how different species’ metabolisms will integrate to reach a desired outcome is a difficult problem that has been studied in great detail using steady-state(More)
Government retains a non-exclusive, paid-up, irrevocable, worldwide 29 license to publish or reproduce the published form of this manuscript, or 30 allow others to do so, for United States Government purposes. 31 peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission.
19 Exometabolomics enables analysis of metabolite utilization of low molecular weight organic 20 substances by soil isolates. Environmentally-based defined media are needed to examine ecologically 21 relevant patterns of substrate utilization. Here, we describe an approach for the construction of defined 22 media using untargeted characterization of water(More)
Mass spectrometry imaging (MSI) has emerged as a powerful technique enabling spatially defined imaging of metabolites within microbial biofilms. Here, we extend this approach to enable differentiation of newly synthesized versus pre‐existing metabolites across a co‐culture. This is accomplished by MS imaging two soil microbes, Shewanella oneidensis MR1 and(More)
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