Learn More
  • R P Henry
  • 2001
The enzyme carbonic anhydrase appears to be a central molecular component in the suite of physiological and biochemical adaptations to low salinity found in euryhaline crustaceans. It is present in high activities in the organs responsible for osmotic and ionic regulation, the gills, and more specifically, the individual gills that are specialized for(More)
Carbonic anhydrase (CA) activity in the gills of the euryhaline blue crab, Callinectes sapidus, was measured in response to acute low-salinity transfer and treatment with eyestalk ablation (ESA) in an attempt to elucidate potential regulatory mechanisms of salinity-mediated CA induction. ESA alone resulted in an approximate doubling of CA activity in the(More)
Carbonic anhydrase (CA) is a central enzyme to both transport and metabolic processes at the cellular level. In metabolically active tissue such as muscle, CA in the cytoplasm and on the sarcolemma appears to be important in facilitating CO2 transport out of the cell. Membrane-associated CA, with an extracellular orientation, also appears to be important in(More)
The crustacean gill is a multi-functional organ, and it is the site of a number of physiological processes, including ion transport, which is the basis for hemolymph osmoregulation; acid-base balance; and ammonia excretion. The gill is also the site by which many toxic metals are taken up by aquatic crustaceans, and thus it plays an important role in the(More)
Reviewers The Publications Committee of the American Physiological Society gratefully acknowledges the services of the following reviewers who assisted the Editorial Board in the reviews of manuscripts. (invited only) have specific instructions that you should review if you are submitting to them. The American Physiological Society (APS) Journals seek(More)
Carcinus maenas, commonly known as the European green crab, is one of the best-known and most successful marine invasive species. While a variety of natural and anthropogenic mechanisms are responsible for the geographic spread of this crab, its ability to adapt physiologically to a broad range of salinities, temperatures and other environmental factors has(More)
The interaction between environmental salinity and gene expression was studied in gills of the euryhaline green shore crab Carcinus maenas. A 4462-feature oligonucleotide microarray was used to analyze changes in transcript abundance in posterior ion-transporting gills at 8 time periods following transfer of animals from 32 to 10 or 15 ppt salinity.(More)
An isolated, perfused tail preparation was used to study the role of carbonic anhydrase (CA) in CO2 and NH3 transport across the sarcolemma of white muscle in the rainbow trout. Tissue was perfused with either control saline or saline containing the CA inhibitors quaternary ammonium sulfanilamide (QAS) or acetazolamide (Az). Inhibition of extracellular CA(More)
Two euryhaline species of decapod crustaceans, Carcinus maenas and Callinectes sapidus, were subjected to a series of acute low-salinity challenges, and changes in carbonic anhydrase (CA) activity in the gills were monitored in order to characterize the nature of salinity-sensitive CA induction. CA activity is uniformly low in all gills of both species at(More)
The time course of induction of activity of carbonic anhydrase (CA) and Na/K ATPase, two enzymes that are central to osmotic and ionic regulation in the eyryhaline green crab, Carcinus maenas, was measured in response to a transfer from 32 to 10 ppt salinity. CA activity was low in all gills in crabs acclimated to high salinity. Activity was induced in the(More)