Raymond J. Robbins

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Twenty-six mother-child dyads played together in a laboratory setting. Play sessions were surreptitiously videotaped (with mothers' permission), and each maternal vocalization was transcribed and coded, first into 1 of 24 categories and then ipso facto into one of three supercategories--namely, controlling, autonomy supportive, and neutral. The degree of(More)
We report that cultured vascular endothelial cells release into the culture medium a vasoconstrictor peptide, a substance we call an endothelium-derived constricting factor (EDCF). Conditioned medium from cultured bovine aortic and pulmonary artery endothelial cells caused sustained, dose-dependent isometric constriction of vascular rings isolated from(More)
Lipoprotein lipase (LP lipase, triacylglycero-protein acylhydrolase EC 3.1.1.34) activity was found in four dissimilar brain regions (hypothalamus, cortex, cerebellum, and midbrain) of adult male rats. Progressive accumulation of LP lipase activity in cultured fetal rat hypothalamic cells was also observed, indicating de novo synthesis of the lipase. The(More)
Picosecond time-dependent fluorescence depolarization techniques have been used to monitor the reorientation of ethidium bromide intercalated in DNA and RNA. The fluorescence polarization anisotropy reveals a nonexponential, exp(-at 1/2), torsional relaxation of the DNA double helix and provides an accurate value for its torsional rigidity, C = 1.3 +/- 0.2(More)
The fluorescence decay kinetics of aqueous tryptophan and 3-methylindole have been determined as a function of pH and temperature by using a picosecond dye laser-single photon counting system with a time resolution of 50 ps. At pH 11, tryptophan exhibits a single exponential decay, with a lifetime of 9.1 ns at 18 O C . However, at pH 7 the decay is faster(More)
Time-resolved fluorescence spectroscopy of tryptophan and two related dipeptides, tryptophylalanine and alanyltryptophan, has been carried out on the subnanosecond time scale by using picosecond exciting pulses at a wavelength of 264 nm. Detection was with an ultrafast streak camera coupled to an optical multichannel analyzer. The zwitterions of these(More)
The hippocampi of species commonly used for in vitro physiologic studies were examined to determine if there were species-specific and regional differences in somatostatin immunoreactivity. The distributions of somatostatin-immunoreactive somata and fiber plexuses were determined, and the concentration of somatostatin along the septotemporal axis of the(More)
Picosecond time-resolved fluorescence studies of 8-anilino-1 -naphthalenesulfonic acid ( A N S ) have been carried out in a water-ethanol mixed-solvent system. Combined with quantum yield results, these data provide further insight into the nonradiative processes that can take place in this interesting fluorescence probe molecule. I n a mixed solvent, the(More)
The use of human fetal CNS tissue for implantation into the brains of patients with neurological disorders and for basic research requires definitive identification and characterization of the donor tissue. The presence of tyrosine hydroxylase (TH)-like immunoreactivity, the levels of various monoamines and their metabolites and the viability of(More)