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Metabolite profiling of industrially important suspension-cultured mammalian cells is being increasingly used for rational improvement of bioprocesses. This requires the generation of global metabolite profiles that cover a broad range of metabolites and that are representative of the cells at the time of sampling. The protocol described here is a validated(More)
The gravity method was the first geophysical technique to be used in oil and gas exploration and, despite being eclipsed by seismology, it has continued to be an important and sometimes crucial additional constraint in a number of exploration areas. In oil exploration the gravity method is particularly applicable in salt provinces, overthrust and foothills(More)
Global metabolite analysis approaches, coupled with sophisticated data analysis and modeling procedures (metabolomics), permit a dynamic read-out of how cellular proteins interact with cellular and environmental conditions to determine cell status. This type of approach has profound potential for understanding, and subsequently manipulating, the regulation(More)
The extended Euler deconvolution algorithm is shown to be a generalization and unification of 2-D Euler de-convolution and Werner deconvolution. After recasting the extended Euler algorithm in a way that suggests a natural generalization to three dimensions, we show that the 3-D extension can be realized using generalized Hilbert transforms. The resulting(More)
Fourier transform infrared (FT-IR) spectroscopy combined with multivariate statistical analyses was investigated as a physicochemical tool for monitoring secreted recombinant antibody production in cultures of Chinese hamster ovary (CHO) and murine myeloma non-secreting 0 (NS0) cell lines. Medium samples were taken during culture of CHO and NS0 cells lines,(More)
Recombinant monoclonal antibodies (MAbs) are increasingly being used for therapeutic use and correct glycosylation of these MAbs is essential for their correct function. Glycosylation profiles are host cell- and antibody class-dependent and can change over culture time and environmental conditions. Therefore, rapid monitoring of glycan addition/status is of(More)
The gravity method was the first geophysical technique to be used in oil and gas exploration. Despite being eclipsed by seismology, it has continued to be an important and sometimes crucial constraint in a number of exploration areas. In oil exploration the gravity method is particularly applicable in salt provinces, overthrust and foothills belts,(More)
The proteome of the anaerobic organohalide-respiring bacterium Dehalococcoides mccartyi strain CBDB1 was analyzed by nano liquid chromatography coupled to mass spectrometry (LC-MS/MS). Two different preparation methods, (i) in-solution and (ii) in-gel proteolytic digestion were assessed to elucidate the core and the functional proteome of bacterial cultures(More)
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