Raphaël Trouillon

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Magnetic micro- and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quantification of a sample's analyte concentration have been among the most sensitive and have allowed(More)
We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive stochastic reorientations in an engineered magnetic energy landscape. The latter consists of a pattern of(More)
In this file of Supporting Information, we provide additional details on • the processing routine for the CV data; • the method used to assess the electrode capacitance from CV background traces; • The numerical values for σ AMP and σ CV (Table S1); • a method to estimate the relaxation time of the diffusion layer through an analogy with electronics; • the(More)
The time response of an electrode incorporated into a fluidic channel to variations in analyte concentration of the outer-sphere redox probe ferrocenemethanol was investigated both for amperometry (AMP) and cyclic voltammetry (CV). The experimental data show that the temporal resolution of CV is not as good as that of AMP, as CV cannot properly detect fast(More)
We demonstrate a method to determine the Brownian motion and the diffusion coefficient of a nanoparticle in water in a plane that is parallel to a solid boundary and as function of the distance normal to that boundary by using an optical tweezers instrument. A solution of 190 nm-diameter fluorescent polystyrene nanoparticles in de-ionized (DI) water is(More)
Fluorescence in situ hybridization (FISH) is one of the recommended techniques for human epidermal growth factor receptor 2 (HER2) status assessment on cancer tissues. Here we develop microfluidics-assisted FISH (MA-FISH), in which hybridization of the FISH probes with their target DNA strands is obtained by applying square-wave oscillatory flows of diluted(More)
1 Member of the ISE The diffusion layer is a critical factor affecting the temporal response of electrochemical devices. In this article, we have investigated the effect of the relaxation of the diffusion layer during the potentiodynamic sensing of ferrocenemethanol (FcMeOH) in microchannels and compared these results to amperometry. First, the effect of(More)
The time responses of two electrochemical methods, amperometry (AMP) and cyclic voltammetry (CV), used in a microchannel to detect the concentration variations of an outer-sphere redox species were compared. Overall, our results show that the temporal resolution of AMP is superior to the one of CV. As no secondary reaction (formation of chemical bounds,(More)
The fluorescence in situ hybridization (FISH) is the gold standard in human epidermal growth factor receptor 2 (HER2) status assessment in breast cancer. The dissemination of the technique is impeded by the cost of reagents and long experimental time. For overcoming these limitations, we have developed a new method for implementing FISH for HER2 assessment(More)