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MicroRNAs (miRNAs) are small endogenous RNAs of ~22 nucleotides that have been shown to play regulatory role by negatively affecting the expression of genes at the post-transcriptional level. Information of miRNAs on some important crops like soybean, Arabidopsis, and rice, etc. are available, but no study on heat-responsive novel miRNAs has yet been(More)
Heat stress causes an array of physiological, biochemical and morphological changes in plants, affecting growth and yield. Here, we report cloning of HSP90 gene of 2,323 bp from C-306 cultivar of wheat having ORF from 62 to 2,164 bp encoded for 700 amino acids. Quantitative real time expression analysis of HSP90 gene in C-306 showed 1.5, 1.2, 2.5 fold (in(More)
Calcium acts as a signaling molecule and plays very important role in plants-tolerance to the abiotic stresses including the heat stress. We studied the effect of exogenous Ca2+ (10 mM) prior to the heat stress (42 °C, 2 h) on different molecular and biochemical parameters associated with thermotolerance in wheat (Triticum aestivum). We could observe 64 and(More)
The reproductive stage of wheat is highly sensitive to the heat stress, especially pollen viability. Here, we report the alterations in the expression of heat shock proteins and antioxidant enzymes in wheat pollen of thermotolerant (C-306) and susceptible (HD2329) cultivars under the heat shock (42 °C, 2 h) with or without ascorbic acid (400 mM) treatment.(More)
RuBisCo activase (Rca) is a catalytic chaperone involved in modulating the activity of RuBisCo (key enzyme of photosynthetic pathway). Here, we identified eight novel transcripts from wheat through data mining predicted to be Rca and cloned a transcript of 1.4 kb from cv. HD2985, named as TaRca1 (GenBank acc. no. KC776912). Single copy number of TaRca1 was(More)
Heat stress is one of the major problems in agriculturally important cereal crops, especially wheat. Here, we have constructed a subtracted cDNA library from the endosperm of HS-treated (42°C for 2 h) wheat cv. HD2985 by suppression subtractive hybridization (SSH). We identified ~550 recombinant clones ranging from 200 to 500 bp with an average size of 300(More)
After cellular uptake, Copper (Cu) ions are transferred from the chaperone Atox1 to the Wilson disease protein (ATP7B) for incorporation into Cu-dependent enzymes in the secretory pathway. Human ATP7B is a large multi-domain membrane-spanning protein which, in contrast to homologues in other organisms, has six similar cytoplasmic metal-binding domains(More)
Heat stress adversely affects growth, development, and yield of winter wheat (Triticum aestivum). Plants have, however, evolved mechanisms to adapt to such conditions mainly by the expression of stress-associated chaperones, the heat shock proteins (HSPs), for modulating the tolerance level. Here, we report cloning of cytosolic putative HSP70 of 1678 bp(More)
High temperature reduces the growth and yield of most of the agriculturally important crops. Elicitors have been reported to modulate the thermotolerance of crops under abiotic stresses. Here, we studied the effect of salicylic acid (SA) on thermotolerance level of C306 (thermotolerant) and PBW343 (thermosusceptible) wheat cultivars grown under heat stress.(More)
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