Randy Hartline

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The ability of bornane and substituted bornanes to induce the early enzymes for d(+)-camphor degradation and control of these enzymes by catabolite repression were studied in a strain of a Pseudomonas putida. Bornane and 20 substituted bornane compounds showed induction. Of these 21 compounds, bornane and 8 of the substituted bornanes provided induction(More)
Two methods for bacterial membrane transport, filtration and flow dialysis, were used to study cellular association of Pseudomonas putida with naphthalene. It is not technically possible to determine the exact cellular or vesicular location of the naphthalene, and because it is hydrophobic, it could be at the membrane(s) rather than inside the cells. As an(More)
In vitro effects of ethanol on calcium taken up by synaptosomes were examined in two strains of mice, C57BL and DBA, that exhibit marked differences in alcohol sensitivity and preference. There were no significant strain differences in basal or depolarization-dependent synaptosomal calcium levels. Ethanol did not reduce the basal calcium level but instead(More)
Baginsky, Marietta L. (University of California, San Francisco Medical Center, San Francisco), and Victor W. Rodwell. Metabolism of pipecolic acid in a Pseudomonas species. IV. Electron transport particle of Pseudomonas putida. J. Bacteriol. 92:424-432. 1966.-Enzymes of Pseudomonas putida P2 catalyzing oxidation of pipecolate to(More)
Free-moving mice from the high-alcohol preference C57BL/6J strain and low-preference DBA/2J strain were slowly fed [2-14C]ethanol intragastrically until anesthesia was achieved. Behavior was monitored in a Plexiglas metabolic chamber while 14CO2 was simultaneously trapped to determine the rate of ethanol metabolism. Average time to the loss of the righting(More)
In the presence of pipecolate, Pseudomonas putida P2 (ATCC 25571) converts cu-amino[6-14C]adipate to radioactive glutamate with a 14C distribution of 11% and 80 % in carbons 1 and 5, respectively. This labeling pattern, when considered with previous data, indicates that a-aminoadipate is an obligate intermediate in the metabolism of pipecolate to glutamate.(More)
The properties of a system for octanoate transport in Pseudomonas oleovarans are described. Transport is inducible and energy dependent, shows saturation kinetics, and concentrates against a gradient. Optimal transport is at pH 6.0 and 28 C. Apparent K(m) and V(max) values are, respectively, 7.0 muM octanoate and 0.68 nmol of octanoate transported per min(More)
Pseudomonas putida (oleovorans) (Pp(OCT)) cured of its OCT plasmid (Pp) no longer grows on D-lysine. Conjugation of PpTrp- with three different methionine auxotrophs carrying the OCT plasmid resulted in PpTrp- (OCT) organisms that grew on D-lysine. Three early D-lysine catabolic enzymes encoded by the OCT plasmid are a lysine racemase, the proposed(More)