Ramasamy Selvam

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Membrane injury facilitated the fixation of calcium oxalate crystals and subsequent growth into kidney stones. Oxalate-induced membrane injury was mediated by lipid peroxidation reaction through the generation of oxygen free radicals. In urolithic rat kidney or oxalate exposed cultured cells, both superoxide anion and hydroxyl radicals were generated in(More)
Renal injury is considered as one of the prerequisites for calcium oxalate retention. In order to determine the role of lipid peroxidation related effects for hyperoxaluria, we evaluated the alterations in lipid peroxidation, antioxidants and oxalate synthesizing enzymes in lithogenic rats with response to vitamin E + selenium treatment. In kidney of(More)
Urolithiasis is one of the third most common afflictions found in humans. The efficacy of the two Siddha drugs, Aerva lanata and Vediuppu chunnam as antilithic agents using a urolithic rat model were tested in this study. Hyperoxaluria was induced in rats using 0.75% ethylene glycol in drinking water. Aerva lanata(3.0 mg kg(-1)body weight) and Vediuppu(More)
OBJECTIVE Urolithiasis and free radicals have long been associated. In this study, we have isolated calcium oxalate monohydrate (COM) binding proteins from rat kidney before and after lipid peroxidation (LPO) and studied its properties on calcium oxalate crystal growth. MATERIALS AND METHODS LPO was carried out using t-butyl hydroperoxide, cumene(More)
Rheumatoid arthritis (RA) is a chronic inflammatory disorder affecting 1% of the population worldwide. Pulsed electromagnetic field (PEMF) has a number of well-documented physiological effects on cells and tissues including antiinflammatory effect. This study aims to explore the antiinflammatory effect of PEMF and its possible mechanism of action in(More)
To understand the gender differences noticed in autoimmune disorders, particularly rheumatoid arthritis, we used a rat model of collagen induced arthritis (CIA). This study was carried out in two parts. In the first study, severity of inflammation was compared between male and female rats with respect to radiology, histology, activities of lysosomal(More)
Kidney stone matrix protein fractions eluted from DEAE cellulose column showed increased oxalate binding activity and had negative correlation with reduced thiol content. Fraction 1 (eluted in Tris-HCl, pH 7.4) and fraction 3 (0.3 M NaCl in buffer) showed nucleation and aggregation-promoting properties while fraction 2 (0.05 M NaCl in buffer) showed an(More)
Biochemical alterations in 152 malaria patients infected with Plasmodium vivax were studied and the effect of parasitaemia on these changes was assessed. The degree of parasitaemia correlated positively with plasma uric acid, total and unconjugated bilirubin. A decrease in the levels of serum total protein, albumin, serum total, free and ester cholesterol(More)
OBJECTIVES To isolate calcium oxalate-binding proteins from human kidney and characterize the functional properties. MATERIALS AND METHODS Calcium oxalate crystals were prepared and allowed to interact at two different pH values with Triton-extracted human kidney homogenate. The proteins in the homogenate were isolated and fractionated on a cellulose(More)
OBJECTIVE To determine the functional role of calcium oxalate binding proteins in the nucleation, aggregation and retention of calcium oxalate crystals under physiological and hyperoxaluric conditions. Materials and methods Hyperoxaluria was induced in rats using 0.75% of ethylene glycol in drinking water. Calcium oxalate binding proteins were isolated and(More)