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Transcription in plastids is mediated by a plastid-encoded multimeric (PEP) and a nuclear-encoded single-subunit RNA polymerase (NEP) and a still unknown number of nuclear-encoded factors. By combining gel filtration and affinity chromatography purification steps, we isolated transcriptionally active chromosomes from Arabidopsis thaliana and mustard(More)
Piriformospora indica, a member of the newly created order Sebacinales, is extremely versatile in its mycorrhizal associations and its ability to promote plant growth. P. indica is widely distributed as a symptomless root endophyte, and it colonizes members of bryophytes, pteridophytes, gymnosperms and angiosperms. P. indica and allied members of(More)
Piriformospora indica, an endophytic fungus of the Sebacina-ceae family, colonizes the roots of a wide variety of plant species and promotes their growth, in a manner similar to arbuscular mycorrhizal fungi. The results of the present study demonstrate that the fungus interacts also with the non-mycorrhizal host Arabidopsis thaliana and promotes its growth.(More)
Piriformospora indica is an endophytic fungus that colonizes roots of many plant species and promotes growth and resistance to certain plant pathogens. Despite its potential use in agriculture, little is known on the molecular basis of this beneficial plant-fungal interaction. In a genetic screen for plants, which do not show a P. indica- induced growth(More)
Verticillium dahliae (Vd) is a soil-borne vascular pathogen which causes severe wilt symptoms in a wide range of plants. The microsclerotia produced by the pathogen survive in soil for more than 15 years. Here we demonstrate that an exudate preparation induces cytoplasmic calcium elevation in Arabidopsis roots, and the disease development requires the(More)
Piriformospora indica association has been reported to increase biotic as well as abiotic stress tolerance of its host plants. We analyzed the beneficial effect of P. indica association on rice seedlings during high salt stress conditions (200 and 300 mM NaCl). The growth parameters of rice seedlings such as root and shoot lengths or fresh and dry weights(More)
More than 100,000 publications demonstrate that AGC kinases are important regulators of growth, metabolism, proliferation, cell divison, survival and apoptosis in mammalian systems. Mutation and/or dysregulation of these kinases contribute to the pathogenesis of many human diseases, including cancer and diabetes. Although AGC kinases are also present in(More)
Mild photooxidative stress combined with water deficit affected the expression of genes of cytokinin (CK) signal transduction. According to qRT-PCR data, these stress factors suppressed the expression of CK receptor genes AHK2 and AHK3 and the ARR5 response regulator gene, and slightly activated the expression of AHK4 gene. The absence of AHK2 and AHK3(More)
Piriformospora indica, an endophytic fungus of Sebacinales, colonizes the roots of many plant species including Arabidopsis thaliana. The symbiotic interaction promotes plant performance, growth and resistance/tolerance against abiotic and biotic stress. We demonstrate that exudated compounds from the fungus activate stress and defense responses in the(More)
Phosphorus (P) is a major macronutrient for plant health and development. The available form of P is generally low in the rhizosphere even in fertile soils. A major proportion of applied phosphate (Pi) fertilizers in the soil become fixed into insoluble, unavailable forms, which restricts crop production throughout the world. Roots possess two distinct(More)