The study of dynamic cellular processes demands appropriate test systems. While static endpoint measurements fail to reflect kinetic variation, a real-time system provides comprehensive insights into cellular migration and invasion processes. Here we present how this method has been applied to study inflammation, and cancer metastasis.
In cell-based research, stringent quality control is desirable for standardization and correct interpretation of results. This article demonstrates the power of real-time cell analysis (RTCA) to achieve this goal using a highly reproducible, yet convenient and comprehensive method.