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Co-transformation of Oryza sativa L. var. Pusa Basmati1 was done using an Agrobacterium tumefaciens strain harbouring a single-copy cointegrate vector and a multi-copy binary vector in the same cell. The T-DNA of the cointegrate vector pGV2260::pSSJ1 carried the hygromycin phosphotransferase (hph) and β-glucuronidase (gus) genes. The binary vector(More)
Phosphorylation of eukaryotic translation initiation factor 2alpha (eIF2alpha) is a common cellular mechanism to limit protein synthesis in stress conditions. Baculovirus PK2, which resembles the C-terminal half of a protein kinase domain, was found to inhibit both human and yeast eIF2alpha kinases. Insect cells infected with wild-type, but not pk2-deleted,(More)
A high copy number, selectable marker gene (SMG)-free Agrobacterium binary vector pBin19ΔnptII was constructed by deleting the nptII gene from pBin19. The binary vectors with the RK2 and pVS replication origins exist in 12 and 3 copies, respectively, in Agrobacterium. The tobacco osmotin gene (ap24) was cloned in pBin19ΔnptII and the resultant plasmid(More)
Rice chitinase (chi11) and tobacco osmotin (ap24) genes, which cause disruption of fungal cell wall and cell membrane, respectively, were stacked in transgenic rice to develop resistance against the sheath blight disease. The homozygous marker-free transgenic rice line CoT23 which harboured the rice chi11 transgene was sequentially re-transformed with a(More)
In an analysis of 339 independent T 0 transgenic rice lines generated by Agrobacterium-mediated transformation, albino plants appeared in the T 1 generation in two single-copy transgenic lines, O54 and O36 and in one double-copy transgenic line, C18. While the T 0 plants of these three lines were green, albino and green plants emerged in a 1:3 ratio in the(More)
Agrobacterium-mediated transformation was used to introduce pathogenesis-related protein genes into scutellum-derived callus of rice (Oryza sativa L. subsp. indica ‘White Ponni’) as a means of increasing resistance to sheath blight disease caused by Rhizoctonia solani Kühn. Transformation with a tobacco osmotin (ap24) gene driven by the Cauliflower mosaic(More)
We report a new T-DNA-tagged rice plant chi7, which displays a severe dwarf phenotype, reduced plant vigour, and impaired panicle development in the homozygous state. By chromosome walking, T-DNA integration was mapped in chromosome 2, 1054-bp upstream of the translation start site of a gene (Os02g0820400), which we designate as DWARF SHOOT AND DEFECTIVE(More)
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