Rahul Ravindra

Learn More
The mechanism of ethanol inhibition of glucose uptake was investigated using C6 glioma cells. Basal [3H]2-deoxy-D-glucose (2DG) uptake by C6 cells was inhibited by ethanol in a concentration-dependent manner. Fifty, 75 and 100 mM ethanol significantly inhibited basal 2DG uptake by 12, 20 and 23%, respectively (p < 0.05). Carbachol (an agonist acting via G(More)
In lactating rats, suckling stimulated the in-vitro tubulin assembly of cold-labile pituitary tubulin fraction by about 70%. Domperidone and alpha methyl-p-tyrosine stimulated the pituitary tubulin assembly by 90% and 325%, respectively. In another experiment, pituitaries from a group of lactating rats (no reference to suckling) were processed to obtain(More)
A [3H]colchicine-binding assay was employed for estimating the relative amounts of soluble and polymerized tubulin present in an individual anterior pituitary lobe. Colchicine binding to these two tubulin pools was time dependent and dose responsive. Scatchard analysis of the binding revealed that [3H]colchicine bound to both fractions with similar(More)
To understand the mechanism of ethanol action on G protein-mediated signal transduction pathway, the effect of ethanol on muscarinic receptor-G protein coupling in the rat cerebral cortex was examined. Acetylcholine (ACh)-stimulated G protein GTPase activity was used as an index of receptor-G protein coupling. ACh stimulation of G protein GTPase activity(More)
To evaluate the role of G proteins in acetylcholine (ACh)-mediated signal transduction, a novel assay to measure [35S]GTP gamma S binding to rat striatal membranes was standardized. ACh and carbamylcholine (CCh) stimulated the high affinity [35S]GTP gamma S binding to striatal membranes by up to 45% in a concentration-dependent manner. A time course study(More)
Plasma membranes from anterior pituitary lobes of rats and GH3 cells were exposed to monoclonal anti-beta tubulin antibodies or normal mouse IgG, and treated with FITC-labeled anti-mouse IgG. Specimens were visualized with a microscope equipped with epifluorescence optics. Tubulin-specific fluorescence was very prominent in membranes incubated with tubulin(More)
The effect of thyrotropin-releasing hormone (TRH) on the tubulin-microtubule system in GH3 cells was investigated. Prolactin (PRL) secretion by GH3 cells was stimulated by TRH at 1, 5, and 10 min of incubation. The polymerized tubulin levels increased from 0.4 units micrograms-1 protein in control cells to 0.6 units micrograms-1 protein in cells incubated(More)
The role of G proteins in glucose uptake was investigated using C6 glioma cells. Carbachol (an agonist acting via G protein coupled receptors) and 5'-guanylylimidodiphosphate (Gpp(NH)p; a nonhydrolysable guanine nucleotide analog which bypasses the receptors and directly activates G proteins) stimulated [3H]2-deoxy-D-glucose (2DG) uptake by C6 cells,(More)
To investigate the possibility that insulin-stimulated glucose uptake in C6 cells is due to transactivation of a G protein-mediated pathway, the role of Gq alpha in insulin signaling was studied. Insulin stimulation of [3H]2-deoxy-D-glucose (2DG) uptake by C6 cells was time- and concentration-dependent: at a concentration of 1 microM, insulin stimulated 2DG(More)