Raffaela Mistò

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BACKGROUND Traditional ABO blood group serology is based on the immunoreactivity of antisera with the carbohydrate A, B and H antigens. Progress in the molecular biology of the ABO system has recognized the molecular basis of the red cell (RBC) antigens and has provided a genetic model for ABO polymorphism at the molecular level. Recently, this genetic(More)
HLA class I typing by standard microcytotoxicity testing has been unsatisfactory for 14.5% of 1644 cord blood samples. In this study, we evaluated the capacity of PCR-SSP in solving problems in HLA-A,B typing with serological methods. With this aim we have compared serology with PCR-SSP in 100 cord blood samples with doubtful or unreliable HLA-A,B typing.(More)
PURPOSE To evaluate the effect of corneal deswelling prior to a microkeratome and femtosecond (fs) laser cut and the impact of the fs laser energy settings on the tissue surface and collagen quality. METHODS Porcine and human corneas were incubated in THIN-C (deswelled) or EUSOL-C (control) at 4°C for 4 hours. Porcine corneas were cut using a(More)
Pregnant albino mice were treated with 5-azacytidine so that the embryonic brains were affected late in their morphological ontogeny. The offspring showed retarded body growth and a conspicuous reduction in the size of the cerebral hemispheres as measured at the end of development. Histological alterations were found in the hippocampus and the cingulate(More)
PURPOSE To describe the case of a donor cornea that showed hematogenous metastatic spread of cutaneous melanoma to the sclerocorneal limbus. METHODS Corneal tissue obtained from a donor with cutaneous malignant melanoma was evaluated for endothelial cell density, corneal transparency, and epithelial morphology. Subsequently, hematoxylin and eosin staining(More)
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