Rafael Lafuente

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OBJECTIVE To compare the quality of sperm samples obtained after density-gradient centrifugation and swim-up by performing a nuclear structural analysis with high magnification microscopy at ×8,000. DESIGN Prospective and randomized split-sample study. SETTING Reproductive Medicine Center. PATIENT(S) Sperm samples from 53 male-partners of couples(More)
Eine kongenitale dyserythropoetische Anämie Typ I wurde bei einem 12jährigen spanischen Knaben diagnostiziert. Auffälligster morphologischer Befund waren Chromatinbrücken zwischen den Kernen getrennter oder zweikerniger Erythroblasten. A new case of congenital dyserythropoietic anemia type I is reported in a 12-year-old Spanish boy. In regard to the(More)
A model system was established to determine whether plasmid transfer occurs in soil and how various environmental conditions and cellular energy states affect the rate of plasmid transfer. Different donor and recipient bacteria were inoculated into sterile sandy lutitic soil microcosms. Dispersion studies were performed with a multipoint inoculator sampler.(More)
UNLABELLED Study Type - Aetiology (case series). LEVEL OF EVIDENCE 4. What's known on the subject? and What does the study add? Oxidative stress seems to be one of the biochemical causes of defective sperm function. Paraoxonases are antioxidant enzymes that degrade lipid peroxides. There is a paucity of data on the possible role played by these enzymes in(More)
Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation for predicting outcome in infertile couples undergoing in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) treatments. A(More)
A case of prolymphocytic leukaemia with immunological characteristics of T-cell type is reported. Three noteworthy findings can be emphasized: the presence of C3 receptors on the T-prolymphocytes, the study of the acid-phosphatase isoenzymatic pattern, which showed an increased band 3 with absence of band 3b, and the morphometric ultrastructural(More)
Isoenzymatic study of leucocytic acid phosphatase under normal conditions identifies 3 isoenzymatic bands, which exhibit a noticeable cell specificity. Band 2 is granulocytic, band 3 lymphocytic and band 4 monocytic in origin. Pathologic deviations in the isoenzymatic pattern are both qualitative and quantitative. For some diseases such as chronic(More)
We report 6 patients with myelodysplastic syndrome, all of whom showed a bizarre nuclear anomaly within the neutrophils that was characterized by extensive clumping of chromatin into large blocks separated by clear zones, generally associated with a lack of segmentation. Anaemia, thrombocytopenia, variable leucocyte counts with leucoerythroblastic picture,(More)