Rafael Gómez-Kosky

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Development of in vitro techniques has enabled rapid clonal propagation, regeneration, and multiplication of genetically manipulated superior clones, production of secondary metabolites, and ex situ conservation of valuable germplasm. This has been possible not only due to the refinements of culture methodologies and applications of cutting-edge areas of(More)
The biomass production of Cymbopogon citratus shoots cultivated in bioreactors according to the temporary immersion (TIS) principle was assessed under different growth conditions. The effect of gassing with CO2-enriched air, reduced immersion frequency, vessel size and culture time on total phenolic and flavonoid content and free radical scavenging effect(More)
The development of somatic embryogenesis in papaya (Carica papaya L.) on semisolid culture medium provides an alternative for propagation and breeding of this species. However, a critical factor in the protocol is the low survival of plants in ex vitro conditions. The aim of this study was to determine the effect of the product phloroglucinol on rooting and(More)
In plants, auxin is distributed directly from cell to cell through polar auxin transport (PAT) by the action of auxin influx (AUX1/LAXs) and efflux (PINs) transporters proteins. In Arabidopsis thaliana, PAT plays an important role in the formation of apical and radicular meristems during somatic embryogenesis (SE). The goal of the present research was to(More)
A critical factor in somatic embryogenesis protocols in papaya (Carica papaya L.) has been incomplete germination of somatic embryos due to formation of a basal callus, which prevents the emission of the radicle. This work aims to achieve complete germination of somatic embryos in liquid and semi-solid culture media. The effect of the culture conditions on(More)
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