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Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
Intracytoplasmic sperm injection in the mouse.
Intracytoplasmic sperm injection was successful in the mouse when a piezo-driven micropipette was used instead of a mechanically driven conventional pipette, and approximately 70% of sperm-injected oocytes developed into blastocysts in vitro.
Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring.
Under the experimental conditions employed, spermatid nuclei were less efficient than testicular sperm nuclei in producing normal offspring, but perhaps this was due to technical rather than inherent problems.
Mice cloned from embryonic stem cells.
- T. Wakayama, I. Rodriguez, A. C. Perry, R. Yanagimachi, P. Mombaerts
- BiologyProceedings of the National Academy of Sciences…
- 21 December 1999
It is shown that late-passage ES cells can be used to produce viable cloned mice and provide a link between the technologies of ES cells and animal cloning, suggesting that it may be possible to clone from a single cell a large number of individuals over an extended period.
Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation
It is concluded that genetic heterozygosity is a crucial parameter for postnatal survival of mice that are entirely derived from ES cells by either nuclear cloning or tetraploid embryo complementation, and it is demonstrated that tetRAploid embryos complementation using F1 ES cells represents a simple, efficient procedure for deriving animals with complex genetic alterations without the need for a chimeric intermediate.
Epigenetic Instability in ES Cells and Cloned Mice
Examination of imprinted gene expression in both mice cloned by nuclear transfer and in the embryonic stem cell donor populations from which they were derived suggests that mammalian development may be rather tolerant to epigenetic aberrations of the genome.
Development of normal mice from oocytes injected with freeze-dried spermatozoa
The results suggest that it may be possible to store the male genomes at room temperature after freeze-drying mouse spermatozoa even after three month preservation in a dried state.
Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa
- H. Kusakabe, M. Szczygieł, D. Whittingham, R. Yanagimachi
- Biology, MedicineProceedings of the National Academy of Sciences…
- 1 November 2001
This procedure was effective for preserving spermatozoa from strains (C57BL/6J, 129/SvJ, and BALB/c) in which the fertility of spermatozosa frozen conventionally is extremely poor.
Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs
The asymmetric density of RCAI receptors across the zona was confirmed by ferritin- RCAI and fluorescein-RCAI labeling of mechanically isolated zonae pellucidae, indicating that the RCAi-binding sites are more densely distributed in the exterior zona regions.
Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei
It is posited that cloned embryos derived from differentiated cell nuclei fail to establish a population of truly pluripotent embryonic cells because of faulty reactivation of key embryonic genes such as Oct4.