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Immunoassay of endogenous plasma insulin in man.
The insulin concentration in plasma has been estimated from the degree of hypoglycemia produced in hypophysectomized, adrenalectomization, alloxan-diabetic rats, and from the increased oxidation of glucose-1-C14 by the rat epididymal fat pad. Expand
Assay of Plasma Insulin in Human Subjects by Immunological Methods
It has been found that human insulin cross-reacts strongly with insulin-binding antibodies in guinea pigs immunized with crystalline beef insulin, and that guinea pig anti-beef insulin serum has characteristics suitable for the detection and measurement of human insulin at concentrations which exist in the plasma of normal fasting subjects. Expand
IMMUNOASSAY OF BOVINE AND HUMAN PARATHYROID HORMONE.
Quantitative aspects of the reaction between insulin and insulin-binding antibody.
In the present studies, experimental data on the reaction between insulin and insulin-binding antibody are tested against several theoretical models and the results are most compatible with a model composed of a univalent insulin reacting with two distinctly different orders of antibody sites. Expand
Immunochemical heterogeneity of parathyroid hormone in plasma.
Findings suggest that parathyroid hormone in human plasma is imbalanced and the apparent disappearance of par Kathyroid hormone was more rapid when measured in antiserum C329 than in antisersum 273 and in both antisera was slower in patients with renal insufficiency than in patientsWith 1° hyperparathyroidism. Expand
Plasma Insulin Concentrations in Nondiabetic and Early Diabetic Subjects: Determinations by a New Sensitive Immuno-assay Technic
During the course of investigations on the metabolism of I-labeled insulin, it was observed that the tagged insulin showed a prolonged retention in the blood stream of all subjects who had received insulin therapy, and it was demonstrated that this behavior was attributable to the presence of insulin-binding antibodies. Expand
Ectopic ACTH production in carcinoma of the lung.
It is suggested that serial plasma ACTH levels may be of value in screening for, and/or management of, patients with carcinoma of the lung, and the incidence of ACTH in extracts of other tumor types was much lower. Expand
Insulin-I131 metabolism in human subjects: demonstration of insulin binding globulin in the circulation of insulin treated subjects.
General principles of radioimmunoassay.