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Identification and Characterization of Bacterial Cutinase*
Cutinase, which exists in both fungi and bacteria, catalyzes the cleavage of the ester bonds of cutin. Fungal cutinases have been extensively studied, however, reports on bacterial cutinases haveExpand
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KpsF Is the Arabinose-5-phosphate Isomerase Required for 3-Deoxy-d-manno-octulosonic Acid Biosynthesis and for Both Lipooligosaccharide Assembly and Capsular Polysaccharide Expression in Neisseria
We have identified and defined the function ofkpsF of Neisseria meningitidis and the homologues of kpsF in encapsulated K1 and K5Escherichia coli. KpsF was shown to be the arabinose-5-phosphateExpand
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Structure and Mechanism of 3-Deoxy-d-manno-octulosonate 8-Phosphate Synthase*
3-Deoxy-d-manno-octulosonate 8-phosphate (KDO8P) synthase catalyzes the condensation of phosphoenolpyruvate (PEP) with arabinose 5-phosphate (A5P) to form KDO8P and inorganic phosphate. KDO8P is theExpand
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Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
BackgroundLipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety,Expand
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Modification of Lipopolysaccharide with Colanic Acid (M-antigen) Repeats in Escherichia coli*
Colanic acid (CA) or M-antigen is an exopolysaccharide produced by many enterobacteria, including the majority of Escherichia coli strains. Unlike other capsular polysaccharides, which have a closeExpand
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New Insights into the Evolutionary Links Relating to the 3-Deoxy-D-arabino-heptulosonate 7-Phosphate Synthase Subfamilies*
Bacterial 3-deoxy-d-arabino-heptulosonate 7-phosphate synthases (DAHPSs) have been divided into either of two classes (Class I/Class II) or subfamilies (AroAIα/AroAIβ). Our investigation into theExpand
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Characterization of N-acetylneuraminic acid synthase isoenzyme 1 from Campylobacter jejuni.
Escherichia coli NeuNAc (N-acetylneuraminic acid) synthase catalyses the condensation of PEP (phosphoenolpyruvate) and ManNAc (N-acetylmannosamine) to form NeuNAc and is encoded by the neuB gene.Expand
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Redefining the requisite lipopolysaccharide structure in Escherichia coli.
Gram-negative bacteria possess an asymmetric lipid bilayer surrounding the cell wall, the outer membrane (OM). The OM inner leaflet is primarily composed of various glycerophospholipids, whereas theExpand
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Substrate and Metal Complexes of 3-Deoxy-d-manno-octulosonate-8-phosphate Synthase from Aquifex aeolicus at 1.9-Å Resolution
3-Deoxy-d-manno-octulosonate-8-phosphate synthase (KDO8PS) from the hyperthermophilic bacteriumAquifex aeolicus differs from its Escherichia coli counterpart in the requirement of a divalent metalExpand
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Crystal structure of the reaction complex of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Thermotoga maritima refines the catalytic mechanism and indicates a new mechanism of allosteric
3-Deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS) catalyzes the first reaction of the aromatic biosynthetic pathway in bacteria, fungi, and plants, the condensation of phosphoenolpyruvateExpand
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