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Arterial Blood Pressure Responses to Cell-free Hemoglobin Solutions and the Reaction with Nitric Oxide*
The results suggest that blood pressure increases observed upon exchange transfusion with cell-free hemoglobin solutions can not be the result of ⋅NO scavenging reactions at the heme, but rather must be due to alternative physiologic mechanisms. Expand
Microvascular and tissue oxygen distribution.
This study investigates how tissue is oxygenated directly from the arteriolar supply on the basis of current results with newly developed optical techniques for the measurement of local intra- and extravascular pO2 by phosphorescence decay and develops a model that links convective and diffusive processes. Expand
Current status of blood substitute research: towards a new paradigm
  • R. Winslow
  • Medicine
  • Journal of internal medicine
  • 1 May 2003
The current status of blood substitute research is moving towards a new paradigm (Minisymposium), and the role of nanofiltration in this transition is under discussion. Expand
Targeted O2 delivery by low-P50 hemoglobin: a new basis for O2 therapeutics.
High O2 affinity appears to be an important property for cell-free hemoglobin solutions for tissue delivery by a new surface-modified, polyethylene glycol-conjugated human hemoglobin. Expand
Pulmonary gas exchange on the summit of Mount Everest.
An integrated picture of human gas exchange at the highest point on earth is constructed for the first time due to the severe arterial hypoxemia, high pH, and extremely low PCO2 of subjects on the summit of Mt. Everest. Expand
NO-mediated activation of heme oxygenase: endogenous cytoprotection against oxidative stress to endothelium.
No is a determinant in the modulation of the activity of heme oxygenase leading to a major resistance of the endothelium to oxidative stress, and the iron chelator deferoxamine (5 mM) completely suppressed the SNP-mediated cytotoxicity and partially attenuated theActivity of he me oxygenase to a level equal to that mediated by SIN-1 and SNAP. Expand
Dissociation of local nitric oxide concentration and vasoconstriction in the presence of cell-free hemoglobin oxygen carriers.
Perivascular NO reduction is similar for all Hb solutions because NO binding affinities are similar; however, effects on vascular resistance are related to the type of molecular modification, molecular volume, and oxygen affinity. Expand
The role of hemoglobin oxygen affinity in oxygen transport at high altitude
  • R. Winslow
  • Biology, Medicine
  • Respiratory Physiology & Neurobiology
  • 30 September 2007
Hemoglobin is involved in the regulation of O(2) transport in two ways: a long-term adjustment in red cell mass is mediated by erythropoietin (EPO), a response to renal oxgyenation. Short-term,Expand
Oxidative-stress response in vascular endothelial cells exposed to acellular hemoglobin solutions.
These studies suggest that 1) the higher rate of oxidation of alpha alpha Hb contributes to the augmented endothelial HO activity, and 2) both heme release and iron-mediated oxygen radical formation are major contributors to endothelial oxidative stress and cytotoxicity generated by the cross-linked hemoglobin. Expand
The role of facilitated diffusion in oxygen transport by cell-free hemoglobins: implications for the design of hemoglobin-based oxygen carriers.
This study provides a conceptual framework for the design of a 'blood substitute' based on mimicking O(2) transport by RBCs to prevent autoregulatory changes in blood flow and pressure. Expand