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A post-docking role for active zone protein Rim
TLDR
It is demonstrated that C. elegans unc-10 mutants lacking Rim are viable, but exhibit behavioral and physiological defects that are more severe than those of Rab3 mutants. Expand
An open form of syntaxin bypasses the requirement for UNC-13 in vesicle priming
TLDR
It is demonstrated that the open form of syntaxin can bypass the requirement for UNC-13 in synaptic vesicle priming, which is likely thatUNC-13 primesaptic vesicles for fusion by promoting the open configuration ofntaxin. Expand
Granulocyte-colony stimulating factor promotes lung metastasis through mobilization of Ly6G+Ly6C+ granulocytes
TLDR
This study shows that metastatic tumors examined overexpress granulocyte-colony stimulating factor (G-CSF), which expands and mobilizes Ly6G+Ly6C+ granulocytes and facilitates their subsequent homing at distant organs even before the arrival of tumor cells. Expand
Defects in synaptic vesicle docking in unc-18 mutants
TLDR
A reduction of docked vesicles at the active zone in unc-18 mutants is found, suggesting that UNC-18 functions, directly or indirectly, as a facilitator of vesicle docking. Expand
Rapid Redistribution of Synaptic PSD-95 in the Neocortex In Vivo
TLDR
The data suggest that individual PSDs compete for PSD-95 and that the kinetic interactions between PSD molecules and PSDs are tuned to regulate PSD size. Expand
UNC-13 and UNC-10/Rim Localize Synaptic Vesicles to Specific Membrane Domains
TLDR
To morphologically define the site of vesicle priming and identify fusion-competent synaptic vesicles, a rapid physical-fixation technique with immunogold staining and high-resolution morphometric analysis at Caenorhabditis elegans neuromuscular junctions is combined. Expand
Preservation of Immunoreactivity and Fine Structure of Adult C. elegans Tissues Using High-pressure Freezing
TLDR
This work instantly immobilized tissue in vitreous ice by freezing living adult animals under high pressure, which provides a means to characterize the localization of endogenous proteins and exogenous proteins, such as the green fluorescent protein (GFP), with respect to subcellular compartments in C. elegans tissues by using postembedding immunogold labeling. Expand
The Caenorhabditis elegans vab-10 spectraplakin isoforms protect the epidermis against internal and external forces
TLDR
The identification of new mutations in a gene known as vab-10, which lead to severe morphogenesis defects, are reported, and it is shown that vAB-10 corresponds to the C. elegans spectraplakin locus and novel insights into the role of this plakin subfamily are revealed. Expand
An Automated Method to Quantify Microglia Morphology and Application to Monitor Activation State Longitudinally In Vivo
TLDR
An automated image analysis method is developed that enables the quantitative assessment of microglia activation state within tissue based solely on cell morphology, and several morphological parameters, including cell perimeter length, cell roundness and soma size, quantitatively distinguish resting versus activated populations ofmicroglia within tissue comparable to traditional immunohistochemistry methods. Expand
A Caspase Cascade Regulating Developmental Axon Degeneration
TLDR
In vitro, it is shown that genetic deletion of Caspase-3 is fully protective against sensory axon degeneration initiated by trophic factor withdrawal, but not injury-induced Wallerian degeneration, and a biochemical cascade from prosurvival Bcl2 family regulators to Caspasase-9, then Caspases-3, and then Cazase-6 is defined. Expand
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