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Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections
TLDR
A multiplex PCR method was designed for the detection of all categories of diarrheagenic Escherichia coli and proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing, enteropathogenic, enterotoxigenic, and diffuse adherent E. coli in stool samples. Expand
Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model.
TLDR
A model was developed to study inhibitors present in feces which prevent the use of PCR for the detection of Helicobacter pylori, and these are complex polysaccharides possibly originating from vegetable material in the diet. Expand
Distribution of Classical and Nonclassical Virulence Genes in Enterotoxigenic Escherichia coli Isolates from Chilean Children and tRNA Gene Screening for Putative Insertion Sites for Genomic Islands †
TLDR
It is proposed that the eatA and etpA genes should be included in characterizations of ETEC isolates in future epidemiological studies to determine their prevalence in other geographical regions. Expand
Multiplex PCR for Diagnosis of Enteric Infections Associated with Diarrheagenic Escherichia coli
TLDR
A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed and enterohemorrhagic E. coli was identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks. Expand
Diagnostic Microbiologic Methods in the GEMS-1 Case/Control Study
TLDR
The portfolio of diagnostic assays used in the GEMS study can be broadly applied in developing countries seeking robust cost-effective methods for enteric pathogen detection. Expand
Long Polar Fimbriae of Enterohemorrhagic Escherichia coli O157:H7 Bind to Extracellular Matrix Proteins
TLDR
Findings suggest that the interaction of Lpf and ECM proteins contributes to the EHEC colonization of the gastrointestinal tract. Expand
Locus of Adhesion and Autoaggregation (LAA), a pathogenicity island present in emerging Shiga Toxin–producing Escherichia coli strains
TLDR
A 86-kb mosaic PAI composed of four modules that encode 80 genes, including novel and known virulence factors associated with adherence and autoaggregation are reported, which are suggested to contribute to the emergence of emerging LEE-negative STEC strains. Expand
Escherichia coli isolates from inflammatory bowel diseases patients survive in macrophages and activate NLRP3 inflammasome.
TLDR
The role of NLRP3 in pro-inflammatory cytokines production and bacterial elimination was determined in macrophages and it was found that bacteria isolated from a given patient were closely related and shared virulence factors; however, strains from different patients were genetically heterogeneous. Expand
Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates
TLDR
Using collections of pathogenic E. coli isolates from Europe and Latin America, it is demonstrated that the different lpfA types are associated with the presence of specific intimin (eae) adhesin variants and, most importantly, that they are found in specific E. Escherichia coli pathotypes. Expand
Molecular epidemiology and genetic diversity of Listeria monocytogenes isolates from a wide variety of ready-to-eat foods and their relationship to clinical strains from listeriosis outbreaks in Chile
TLDR
It is shown that Chilean isolates of L. monocytogenes from RTE and raw food products can cause disease in humans, representing a public health risk that justifies permanent surveillance. Expand
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