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Elicitor signal transduction leading to production of plant secondary metabolites.
Progress made on several aspects of elicitor signal transduction leading to production of plant secondary metabolites are summarized, including the integration of multiple signaling pathways into or by transcription factors, as well as the linkage of the above signal components in eliciting network through protein phosphorylation and dephosphorylation.
The Catharanthus alkaloids: pharmacognosy and biotechnology.
Despite the availability of only a limited number of biosynthetic genes, the research on C. roseus has already led to a broad scientific spin-off and it is clear that many interesting results can be expected when more genes become available.
NMR-based metabolomic analysis of plants
This protocol is particularly suited for the analysis of secondary metabolites such as phenolic compounds and for primary metabolites, and is rapid; it takes not more than 30 min for sample preparation and a further 10 min for NMR spectrum acquisition.
Biotechnology for the production of plant secondary metabolites
Metabolic engineering seems a promising approach to improve the production of a cell factory and can be used in the cell culture, in the plant itself or even production in other plant species or organisms.
Natural deep eutectic solvents as new potential media for green technology.
Based on the results the novel NADES may be expected as potential green solvents at room temperature in diverse fields of chemistry.
The seco-iridoid pathway from Catharanthus roseus
expression of the eight genes encoding this pathway, together with two genes boosting precursor formation and two downstream alkaloid biosynthesis genes, in an alternative plant host allows the heterologous production of the complex MIA strictosidine, which confirms the functionality of all enzymes of the pathway and highlights their utility for synthetic biology programmes.
Catharanthus terpenoid indole alkaloids: biosynthesis and regulation
Most of the work focussed on early steps of the pathway, particularly the discovery of the 2-C-methyl-d-erythritol 4-phosphate (MEP)-pathway leading to terpenoids, and much progress has been made at alkaloid regulatory level.
Geraniol 10‐hydroxylase1, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis
The stress hormone methyljasmonate strongly induced G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in a C. roseus cell culture, and degenerate PCR primers based on amino acid sequence information cloned the corresponding cDNA.
Cultivation of medicinal and aromatic plants for specialty industrial materials
Specialty materials such as essential oils, pharmaceuticals, colorants, dyes, cosmetics and biocides are obtained from plants. Many species of medicinal and aromatic plants (MAPs) are cultivated for
NMR-based plant metabolomics: where do we stand, where do we go?
Public databases for storing and sharing information are still lacking for NMR-based metabolomic analysis in plants and such databases are urgently needed to make metabolic profiling a real omics technology.