Elicitor signal transduction leading to production of plant secondary metabolites.
Natural deep eutectic solvents as new potential media for green technology.
NMR-based metabolomic analysis of plants
This protocol is particularly suited for the analysis of secondary metabolites such as phenolic compounds and for primary metabolites, and is rapid; it takes not more than 30 min for sample preparation and a further 10 min for NMR spectrum acquisition.
The Catharanthus alkaloids: pharmacognosy and biotechnology.
- R. van der Heijden, D. I. Jacobs, W. Snoeijer, D. Hallard, R. Verpoorte
- Chemistry, BiologyCurrent Medicinal Chemistry
- 29 February 2004
Despite the availability of only a limited number of biosynthetic genes, the research on C. roseus has already led to a broad scientific spin-off and it is clear that many interesting results can be expected when more genes become available.
Biotechnology for the production of plant secondary metabolites
Metabolic engineering seems a promising approach to improve the production of a cell factory and can be used in the cell culture, in the plant itself or even production in other plant species or organisms.
The seco-iridoid pathway from Catharanthus roseus
expression of the eight genes encoding this pathway, together with two genes boosting precursor formation and two downstream alkaloid biosynthesis genes, in an alternative plant host allows the heterologous production of the complex MIA strictosidine, which confirms the functionality of all enzymes of the pathway and highlights their utility for synthetic biology programmes.
Catharanthus terpenoid indole alkaloids: biosynthesis and regulation
Most of the work focussed on early steps of the pathway, particularly the discovery of the 2-C-methyl-d-erythritol 4-phosphate (MEP)-pathway leading to terpenoids, and much progress has been made at alkaloid regulatory level.
Molecular Cloning and Analysis of Strictosidine β-d-Glucosidase, an Enzyme in Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus *
- A. Geerlings, Matias Martinez-Lozano Ibañez, J. Memelink, R. van der Heijden, R. Verpoorte
- Biology, ChemistryJournal of Biological Chemistry
- 4 February 2000
Results show that coordinate induction of the biosynthetic genes forms at least part of the mechanism for the methyl jasmonate-induced increase in TIA production, and new insight in SGD localization has significant implications for the understanding of the complex intracellular trafficking of metabolic intermediates during TIA biosynthesis.
Chalcone synthase and its functions in plant resistance
This review will discuss CHS and its function in plant resistance and its role in salicylic acid defense pathway.