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Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.)
TLDR
Results show that with the software tool developed, EST databases can be efficiently exploited for the development of cDNA-SSRs, EST-derived SSRs are significantly less polymorphic than those derived from genomic regions, a considerable portion of the developed SSRs can be transferred to related species, and compared to RFLP-markers, c DNA- SSRs yield similar patterns of genetic diversity.
Genic microsatellite markers in plants: features and applications.
TLDR
Applications and potential uses of EST-SSRs in plant genetics and breeding could prove useful for marker-assisted selection, especially when the markers reside in the genes responsible for a phenotypic trait.
In silico analysis on frequency and distribution of microsatellites in ESTs of some cereal species.
TLDR
When all these SSRs were analysed for assessing their potential to develop new markers, unique primer pairs could be designed for 30% to 70% of the total non-redundant microsatellites which are up to 3% of total ESTs in the studied species.
Molecular markers and their applications in wheat breeding
TLDR
This review attempts to give an account of different molecular markers currently available for genome mapping and for tagging different traits in wheat, including restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), sequence-tagged sites (STS), DNA amplification fingerprinting (DAF), amplified fragment length SNP (AFLPs) and microsatellites (STMS).
Recent history of artificial outcrossing facilitates whole-genome association mapping in elite inbred crop varieties
TLDR
It is demonstrated that, after accounting for population substructure, the level of LD exhibited in elite northwest European barley can be effectively exploited to map traits by using whole-genome association scans with several hundred to thousands of biallelic SNPs.
AFLP-based molecular characterization of an elite germplasm collection of Jatropha curcas L., a biofuel plant.
TLDR
Molecular diversity estimated in the present study combined with the datasets on other morphological/agronomic traits will be very useful for selecting the appropriate accessions for plant improvement through conventional as well as molecular breeding approaches.
Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers
TLDR
This reference genome sequence will facilitate the identification of the genetic basis of agronomically important traits, and accelerate the development of improved pigeonpea varieties that could improve food security in many developing countries.
Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species
TLDR
The study provides an insight about the frequency and distribution of SSRs in coffee transcriptome, and also demonstrates the successful development of genic-SSRs.
Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement
TLDR
This work reports the ∼738-Mb draft whole genome shotgun sequence of CDC Frontier, a kabuli chickpea variety, which contains an estimated 28,269 genes, and identifies targets of both breeding-associated genetic sweeps and breeding- associated balancing selection.
The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat
TLDR
The results demonstrate the utility of microsatellite markers for detecting polymorphism leading to genotype identification and for estimating genetic diversity among wheat genotypes.
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