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Advanced methods of microscope control using μManager software.
A guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging. Expand
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The Molecular Motor Toolbox for Intracellular Transport
  • R. Vale
  • Biology, Medicine
  • Cell
  • 21 February 2003
Eukaryotic cells create internal order by using protein motors to transport molecules and organelles along cytoskeletal tracks. Recent genomic and functional studies suggest that five cargo-carryingExpand
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Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
Axoplasm from the squid giant axon contains a soluble protein translocator that induces movement of microtubules on glass, latex beads on microtubules, and axoplasmic organelles on microtubules. WeExpand
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A structural change in the kinesin motor protein that drives motility
Kinesin motors power many motile processes by converting ATP energy into unidirectional motion along microtubules. The force-generating and enzymatic properties of conventional kinesin have beenExpand
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Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
Eukaryotic cells store neutral lipids in cytoplasmic lipid droplets enclosed in a monolayer of phospholipids and associated proteins. These dynamic organelles serve as the principal reservoirs forExpand
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Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle
EB1 is an evolutionarily conserved protein that localizes to the plus ends of growing microtubules. In yeast, the EB1 homologue (BIM1) has been shown to modulate microtubule dynamics and linkExpand
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Regulators of the cytoplasmic dynein motor
Eukaryotic cells use cytoskeletal motor proteins to transport many different intracellular cargos. Numerous kinesins and myosins have evolved to cope with the various transport needs that have arisenExpand
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Single-Molecule Analysis of Dynein Processivity and Stepping Behavior
Cytoplasmic dynein, the 1.2 MDa motor driving minus-end-directed motility, has been reported to move processively along microtubules, but its mechanism of motility remains poorly understood. Here,Expand
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Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast
The cytoplasmic localization of messenger RNA creates an asymmetric distribution of proteins that specify cell fate during development in multicellular eukaryotes. The protein Ash1 is a cell-fateExpand
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Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase
During anaphase identical sister chromatids separate and move towards opposite poles of the mitotic spindle. In the spindle, kinetochore microtubules have their plus ends embedded in the kinetochoreExpand
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