Computer Control of Microscopes Using µManager
- A. Edelstein, Nenad Amodaj, Karl Hoover, R. Vale, N. Stuurman
- Computer ScienceCurrent Protocols in Molecular Biology
- 1 October 2010
This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
Advanced methods of microscope control using μManager software.
- A. Edelstein, Mark A. Tsuchida, Nenad Amodaj, Henry Pinkard, R. Vale, N. Stuurman
- Computer ScienceJournal of Biological Methods
- 7 November 2014
A guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.
The Molecular Motor Toolbox for Intracellular Transport
- R. Vale
- 21 February 2003
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
A structural change in the kinesin motor protein that drives motility
A large conformational change of a ∼15-amino-acid region (the neck linker) in kinesin is detected and visualized using electron paramagnetic resonance, fluorescence resonance energy transfer, pre-steady state kinetics and cryo-electron microscopy.
Molecular requirements for actin-based lamella formation in Drosophila S2 cells
The results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells, revealing a novel function of this protein complex in SCAR stability.
Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
It is shown by means of a genome-wide RNA interference screen in Drosophila S2 cells that about 1.5% of all genes function in lipid-droplet formation and regulation, and a subset of the Arf1–COPI vesicular transport proteins also regulated droplet morphology and lipid utilization, thereby identifying a previously unrecognized function for this machinery.
Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes
Dynein is largely inactive in the cytoplasm and that a variety of adapter proteins activate processive motility by linking dynactin to dynein only when the motor is bound to its proper cargo.
Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells
Using a full-genome RNA interference screen of Drosophila S2 cells, about 200 genes that contribute to spindle assembly are identified, more than half of which were unexpected.