• Publications
  • Influence
Computer Control of Microscopes Using µManager
This unit provides step‐by‐step protocols describing how to get started working with µManager, as well as some starting points for advanced use of the software.
Advanced methods of microscope control using μManager software.
A guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.
The Molecular Motor Toolbox for Intracellular Transport
  • R. Vale
  • Biology, Medicine
  • 21 February 2003
Remarkably, fungi, parasites, plants, and animals have distinct subsets of Toolbox motors in their genomes, suggesting an underlying diversity of strategies for intracellular transport.
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
The partial purification of a protein from squid giant axons and optic lobes that induces microtubule-based movements is reported and there is a homologous protein in bovine brain, for which the name kinesin is proposed.
A structural change in the kinesin motor protein that drives motility
A large conformational change of a ∼15-amino-acid region (the neck linker) in kinesin is detected and visualized using electron paramagnetic resonance, fluorescence resonance energy transfer, pre-steady state kinetics and cryo-electron microscopy.
Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells
Using a full-genome RNA interference screen of Drosophila S2 cells, about 200 genes that contribute to spindle assembly are identified, more than half of which were unexpected.
Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle
These results reveal crucial roles for EB1 in mitosis, which is postulate involves its ability to promote the growth and interactions of microtubules within the central spindle and at the cell cortex.
Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
It is shown by means of a genome-wide RNA interference screen in Drosophila S2 cells that about 1.5% of all genes function in lipid-droplet formation and regulation, and a subset of the Arf1–COPI vesicular transport proteins also regulated droplet morphology and lipid utilization, thereby identifying a previously unrecognized function for this machinery.
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
The SunTag provides a versatile platform for multimerizing proteins on a target protein scaffold and is likely to have many applications in imaging and controlling biological outputs.
Molecular requirements for actin-based lamella formation in Drosophila S2 cells
The results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells, revealing a novel function of this protein complex in SCAR stability.