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A new generation of Ca2+ indicators with greatly improved fluorescence properties.
A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylateExpand
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Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Fluorescent proteins are genetically encoded, easily imaged reporters crucial in biology and biotechnology. When a protein is tagged by fusion to a fluorescent protein, interactions betweenExpand
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The green fluorescent protein.
  • R. Tsien
  • Biology, Medicine
  • Annual review of biochemistry
  • 1 July 1998
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins inExpand
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A monomeric red fluorescent protein
All coelenterate fluorescent proteins cloned to date display some form of quaternary structure, including the weak tendency of Aequorea green fluorescent protein (GFP) to dimerize, the obligateExpand
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Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Important Ca2+ signals in the cytosol and organelles are often extremely localized and hard to measure. To overcome this problem we have constructed new fluorescent indicators for Ca2+ that areExpand
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A guide to choosing fluorescent proteins
The recent explosion in the diversity of available fluorescent proteins (FPs) promises a wide variety of new tools for biological imaging. Expand
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Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells
Many proteins associated with the plasma membrane are known to partition into submicroscopic sphingolipid- and cholesterol-rich domains called lipid rafts, but the determinants dictating thisExpand
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Reducing the Environmental Sensitivity of Yellow Fluorescent Protein
Yellow mutants of the green fluorescent protein (YFP) are crucial constituents of genetically encoded indicators of signal transduction and fusions to monitor protein-protein interactions. However,Expand
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Improved green fluorescence
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Identification of a signal for rapid export of proteins from the nucleus
Active nuclear import of protein is controlled by nuclear localization signals (NLSs), but nuclear export is not understood well. Nuclear trafficking of the catalytic (C) subunit of cAMP-dependentExpand
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